Rosenberg, Naomi and Baltimore, David (1976) A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. Journal of Experimental Medicine, 143 (6). pp. 1453-1463. ISSN 0022-1007. PMCID PMC2190217. doi:10.1084/jem.143.6.1453. https://resolver.caltech.edu/CaltechAUTHORS:ROSjem76
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Abstract
A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction.
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| Additional Information: | © 1976 by Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 9 February 1976. We are grateful to Dr. P.C. Kung for his advice on the use of bovine serum albumin gradients. Supported by grant no. VC-4G from the American Cancer Society, a contract from the Virus Cancer Program, and a grant no. CA-14051 from the National Cancer Institute. [N.R. was a] Postdoctoral fellow of the Massachusetts Division of the American Cancer Society. [D.B. was an] American Cancer Society Professor of Microbiology. | |||||||||
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| Issue or Number: | 6 | |||||||||
| PubMed Central ID: | PMC2190217 | |||||||||
| DOI: | 10.1084/jem.143.6.1453 | |||||||||
| Record Number: | CaltechAUTHORS:ROSjem76 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:ROSjem76 | |||||||||
| Usage Policy: | RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. | |||||||||
| ID Code: | 10677 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Archive Administrator | |||||||||
| Deposited On: | 03 Jun 2008 | |||||||||
| Last Modified: | 08 Nov 2021 21:10 |
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