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A conserved strategy for inducing appendage regeneration

Abrams, Michael J. and Tan, Fayth Hui and Basinger, Ty and Heithe, Martin and Li, Yutian and Raffiee, Misha and Leahy, Patrick and Dabiri, John O. and Gold, David A. and Goentoro, Lea A. (2020) A conserved strategy for inducing appendage regeneration. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20201123-131615362

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Abstract

Can limb regeneration be induced? Few have pursued this question, and an evolutionarily conserved strategy has yet to emerge. This study reports a strategy for inducing regenerative response in appendages, which works across three species that span the animal phylogeny. In Cnidaria, the frequency of appendage regeneration in the moon jellyfish Aurelia was increased by feeding with the amino acid L-leucine and the growth hormone insulin. In insects, the same strategy induced tibia regeneration in adult Drosophila. Finally, in mammals, L-leucine and sucrose administration induced digit regeneration in adult mice, including dramatically from mid- phalangeal amputation. The conserved effect of L-leucine and insulin/sugar suggests a key role for energetic parameters in regeneration induction. The simplicity by which nutrient supplementation can induce appendage regeneration provides a testable hypothesis across animals.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2020.11.21.392720DOIDiscussion Paper
ORCID:
AuthorORCID
Abrams, Michael J.0000-0003-1864-1706
Tan, Fayth Hui0000-0002-2160-5311
Dabiri, John O.0000-0002-6722-9008
Gold, David A.0000-0003-0135-4022
Goentoro, Lea A.0000-0002-3904-0195
Alternate Title:Amino acid and sugar supplement induces appendage regeneration in cnidarian, insect, and mammal
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This version posted November 22, 2020. The authors thank Kiersten Darrow and Michael Schaadt at the Cabrillo Marine Aquarium, Cabrillo, CA for the gifts of Aurelia polyps and help with jellyfish culture, Matthew Hunt from the Kavli Nanoscience Institute at Caltech for help with SEM imaging, James McGehee, Angela Stathopolous, and Kai Zinn for sharing Drosophila strains, Yujing Yang and Long Cai for help with imaging, and Carlos Lois for demonstration of digit amputation. We thank Gertrud Schupbach, Natalie Andrew, Anish Sarma, and Aki Ohdera for comments on the manuscript; and Bruce Hay, Dianne Newman, Katalina Fejes-Toth, Luis del Peso, Aditya Saxena, Joe Parker, Marta Truchado Garcia, and Justin Bois for discussion. This work was supported by the National Science Foundation Graduate Research Fellowship Program (1144469; to M.J.A.), the James E. and Charlotte Fedde Cordes Postdoctoral Fellowship in Biology (to D.A.G.); the James S. McDonnell Foundation for Complex Systems Science (220020365; to L.G.), the Center for Environmental Microbial Interactions at Caltech, and Charles Trimble and Caltech’s Biology and Biological Chair’s Council Inducing Regeneration Fund (to L.G.). Author contributions: M.J.A., F.T., and L.G. conceptualize the paper. M.J.A. and T.B. designed and performed experiments and data analysis in Aurelia. F.T. and M.H. designed and performed experiments and data analysis in Drosophila and mouse. Y.L designed and performed experiments and data analysis in a second Drosophila strain. M.R., J.O.D., and D.A.G. contributed experiments in Aurelia and discussion into the study. P.L. contributed help with Aurelia experiments in the natural habitat. M.J.A., F.T., and L.G. wrote the manuscript draft. All authors reviewed and edited the manuscript. The authors have declared no competing interest.
Group:Kavli Nanoscience Institute, Caltech Center for Environmental Microbial Interactions (CEMI)
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipDGE-1144469
James S. McDonnell Foundation220020365
Caltech Center for Environmental Microbial Interactions (CEMI)UNSPECIFIED
James E. and Charlotte Fedde Cordes Postdoctoral FellowshipUNSPECIFIED
Caltech Division of Biology and Biological EngineeringUNSPECIFIED
Charles TrimbleUNSPECIFIED
Subject Keywords:Regeneration, appendage, limb, amino acid, leucine, sugar, insulin, jellyfish, Drosophila, mouse
Record Number:CaltechAUTHORS:20201123-131615362
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201123-131615362
Official Citation:A conserved strategy for inducing appendage regeneration. Michael J Abrams, Fayth Hui Tan, Ty Basinger, Martin L Heithe, Yutian Li, Misha Raffiee, Patrick Leahy, John O Dabiri, David A Gold, Lea A Goentoro. bioRxiv 2020.11.21.392720; doi: https://doi.org/10.1101/2020.11.21.392720
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106788
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Nov 2020 22:26
Last Modified:23 Nov 2020 22:26

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