Kozak, John J. and Gray, Harry B. (2020) Conjecture on the Design of Helical Proteins. Journal of Physical Chemistry B, 124 (49). pp. 11067-11071. ISSN 1520-6106. PMCID PMC8086193. doi:10.1021/acs.jpcb.0c05669. https://resolver.caltech.edu/CaltechAUTHORS:20201124-122000714
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Abstract
In an important advance in our understanding of protein folding, Wolynes and Onuchic found that the frustration ratio, T_f/T_s, for funneled energy landscapes is T_f/T_s ∼1.6. In our recent work on four heme proteins, we showed that when a protein unfolds from the native state to an early unfolded state, the degree of departure is characterized by a ratio f ∼1.6, where f is a measure of the elongation of n-residue segments of the polypeptide chain. Our analysis, which accounts for this apparent similarity in calculated signatures, is based on a logistic-map model of unfolding. We offer an important take home for the de novo protein synthesis community: in order to increase the probability of obtaining good quality crystals, nearest-neighbor repulsive interactions between adjacent residues (or sequences of residues) in the polypeptide chain must be propagated correctly.
| Item Type: | Article | |||||||||
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| Additional Information: | © 2020 American Chemical Society. Received: June 22, 2020; Revised: October 31, 2020; Published: November 24, 2020. The authors thank R. A. Garza-López for helpful discussions. Work at Caltech was supported by the NIH (DK019038) and the Arnold and Mabel Beckman Foundation. The authors declare no competing financial interest. | |||||||||
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| Issue or Number: | 49 | |||||||||
| PubMed Central ID: | PMC8086193 | |||||||||
| DOI: | 10.1021/acs.jpcb.0c05669 | |||||||||
| Record Number: | CaltechAUTHORS:20201124-122000714 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201124-122000714 | |||||||||
| Official Citation: | Conjecture on the Design of Helical Proteins. John J. Kozak and Harry B. Gray. The Journal of Physical Chemistry B 2020 124 (49), 11067-11071; DOI: 10.1021/acs.jpcb.0c05669 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 106814 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 24 Nov 2020 20:43 | |||||||||
| Last Modified: | 12 Feb 2022 00:26 |
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