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Conjecture on the Design of Helical Proteins

Kozak, John J. and Gray, Harry B. (2020) Conjecture on the Design of Helical Proteins. Journal of Physical Chemistry B, 124 (49). pp. 11067-11071. ISSN 1520-6106. PMCID PMC8086193. doi:10.1021/acs.jpcb.0c05669.

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In an important advance in our understanding of protein folding, Wolynes and Onuchic found that the frustration ratio, T_f/T_s, for funneled energy landscapes is T_f/T_s ∼1.6. In our recent work on four heme proteins, we showed that when a protein unfolds from the native state to an early unfolded state, the degree of departure is characterized by a ratio f ∼1.6, where f is a measure of the elongation of n-residue segments of the polypeptide chain. Our analysis, which accounts for this apparent similarity in calculated signatures, is based on a logistic-map model of unfolding. We offer an important take home for the de novo protein synthesis community: in order to increase the probability of obtaining good quality crystals, nearest-neighbor repulsive interactions between adjacent residues (or sequences of residues) in the polypeptide chain must be propagated correctly.

Item Type:Article
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URLURL TypeDescription CentralArticle
Gray, Harry B.0000-0002-7937-7876
Additional Information:© 2020 American Chemical Society. Received: June 22, 2020; Revised: October 31, 2020; Published: November 24, 2020. The authors thank R. A. Garza-López for helpful discussions. Work at Caltech was supported by the NIH (DK019038) and the Arnold and Mabel Beckman Foundation. The authors declare no competing financial interest.
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Arnold and Mabel Beckman FoundationUNSPECIFIED
Issue or Number:49
PubMed Central ID:PMC8086193
Record Number:CaltechAUTHORS:20201124-122000714
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Official Citation:Conjecture on the Design of Helical Proteins. John J. Kozak and Harry B. Gray. The Journal of Physical Chemistry B 2020 124 (49), 11067-11071; DOI: 10.1021/acs.jpcb.0c05669
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106814
Deposited By: Tony Diaz
Deposited On:24 Nov 2020 20:43
Last Modified:12 Feb 2022 00:26

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