Varshavsky, A. Ya. and Ilyin, Yu. V. and Kadyckov, V. I. and Senchenkov, E. P. (1971) Collapse of extended deoxyribonucleoprotein molecules upon increase of the ionic strength of solution. Biochimica et Biophysica Acta, 246 (3). pp. 583-588. ISSN 0005-2787. doi:10.1016/0005-2787(71)90797-0. https://resolver.caltech.edu/CaltechAUTHORS:20201202-154043608
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20201202-154043608
Abstract
The increase of the ionic strength of extremely dilute deoxyribonucleo protein (DNP) solution from 0.001 to 0.1 results in an intramolecular structural transition, which is reflected by a 2.5-fold increase of the sedimentation coefficient of the DNP and by the electron microscopic appearance of DNP molecules. The rate of this structural transition is considerably higher at 37° than at 0°. Both sedimentation and electron microscopic data allow one to suggest the existence of a supercoiled DNA in collapsed DNP molecules. Removal of the very lysine-rich histone (F1) and of some nonhistone proteins from the DNP results in the loss of its ability to undergo the above structural transition.
| Item Type: | Article | ||||||
|---|---|---|---|---|---|---|---|
| Related URLs: |
| ||||||
| ORCID: |
| ||||||
| Additional Information: | © 1971 Published by Elsevier. (Received July 30th, 1971) We are greatly indebted to Professor G.P. Georgiev for support and discussions throughout this work. Thanks are also due to Drs. A.S. Tichonenko and N.E. Gluhova for advice on electron microscopy and to Dr. U.N. Mickelsaar for collaboration in the early stages of this work. | ||||||
| Issue or Number: | 3 | ||||||
| DOI: | 10.1016/0005-2787(71)90797-0 | ||||||
| Record Number: | CaltechAUTHORS:20201202-154043608 | ||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201202-154043608 | ||||||
| Official Citation: | A.Ya. Varshavsky, Yu.V. Ilyin, V.I. Kadyckov, E.P. Senchenkov, Collapse of extended deoxyribonucleoprotein molecules upon increase of the ionic strength of solution, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Volume 246, Issue 3, 1971, Pages 583-588, ISSN 0005-2787, https://doi.org/10.1016/0005-2787(71)90797-0. | ||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
| ID Code: | 106883 | ||||||
| Collection: | CaltechAUTHORS | ||||||
| Deposited By: | George Porter | ||||||
| Deposited On: | 03 Dec 2020 18:33 | ||||||
| Last Modified: | 16 Nov 2021 18:57 |
Repository Staff Only: item control page
