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Collapse of extended deoxyribonucleoprotein molecules upon increase of the ionic strength of solution

Varshavsky, A. Ya. and Ilyin, Yu. V. and Kadyckov, V. I. and Senchenkov, E. P. (1971) Collapse of extended deoxyribonucleoprotein molecules upon increase of the ionic strength of solution. Biochimica et Biophysica Acta, 246 (3). pp. 583-588. ISSN 0005-2787. https://resolver.caltech.edu/CaltechAUTHORS:20201202-154043608

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Abstract

The increase of the ionic strength of extremely dilute deoxyribonucleo protein (DNP) solution from 0.001 to 0.1 results in an intramolecular structural transition, which is reflected by a 2.5-fold increase of the sedimentation coefficient of the DNP and by the electron microscopic appearance of DNP molecules. The rate of this structural transition is considerably higher at 37° than at 0°. Both sedimentation and electron microscopic data allow one to suggest the existence of a supercoiled DNA in collapsed DNP molecules. Removal of the very lysine-rich histone (F1) and of some nonhistone proteins from the DNP results in the loss of its ability to undergo the above structural transition.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0005-2787(71)90797-0DOIArticle
ORCID:
AuthorORCID
Varshavsky, A. Ya.0000-0002-4011-258X
Additional Information:© 1971 Published by Elsevier. (Received July 30th, 1971) We are greatly indebted to Professor G.P. Georgiev for support and discussions throughout this work. Thanks are also due to Drs. A.S. Tichonenko and N.E. Gluhova for advice on electron microscopy and to Dr. U.N. Mickelsaar for collaboration in the early stages of this work.
Issue or Number:3
Record Number:CaltechAUTHORS:20201202-154043608
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201202-154043608
Official Citation:A.Ya. Varshavsky, Yu.V. Ilyin, V.I. Kadyckov, E.P. Senchenkov, Collapse of extended deoxyribonucleoprotein molecules upon increase of the ionic strength of solution, Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, Volume 246, Issue 3, 1971, Pages 583-588, ISSN 0005-2787, https://doi.org/10.1016/0005-2787(71)90797-0.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:106883
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:03 Dec 2020 18:33
Last Modified:03 Dec 2020 18:33

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