Varshavsky, A. J. and Bakayev, V. V. and Chumackov, P. M. and Georgiev, G. P. (1976) Minichromosome of simian virus 40: presence of histone HI. Nucleic Acids Research, 3 (8). pp. 2101-2114. ISSN 0305-1048. PMCID PMC343065. doi:10.1093/nar/3.8.2101. https://resolver.caltech.edu/CaltechAUTHORS:20201216-124003102
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Abstract
In contrast to conclusions of previous studies /I–3/ claiming the absence of histone HI from the SV40 and polyoma viral minichromosomes we have found that a preparation of purified SV40 minichromosomes does contain histone HI. The content of HI in relation to other four histones in the SV40 minichromosomes is close to that in the cellular chromatin. Histone HI in the isolated SV40 minichromosomes is bound apparently to internucleosomal DNA stretches as was shown already for HI in the cellular chromatin /4/. In addition it was found that more than 90% of the purified SV40 minichromosomes migrated as a single discrete deoxyribonucleoprotein band upon agarose gel electrophoresis.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1976 Information Retrieval Limited. Received 18 June 1976. We thank T. G. Bakayeva and M. Shifman for their generous collaboration in some of experiments. | |||||||||
| Issue or Number: | 8 | |||||||||
| PubMed Central ID: | PMC343065 | |||||||||
| DOI: | 10.1093/nar/3.8.2101 | |||||||||
| Record Number: | CaltechAUTHORS:20201216-124003102 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20201216-124003102 | |||||||||
| Official Citation: | A.J. Varshavsky, V.V. Bakayev, P.M. Chumackov, G.P. Georgiev, Minichromosome of simian virus 40: presence of histone HI, Nucleic Acids Research, Volume 3, Issue 8, 1 August 1976, Pages 2101–2114, https://doi.org/10.1093/nar/3.8.2101 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 107129 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 16 Dec 2020 21:22 | |||||||||
| Last Modified: | 16 Nov 2021 18:59 |
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