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Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein

Varshavsky, A. J. and Nedospasov, S. A. and Bakayev, V. V. and Bakayeva, T. G. and Georgiev, G. P. (1977) Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein. Nucleic Acids Research, 4 (8). pp. 2725-2746. ISSN 0305-1048. PMCID PMC342604. https://resolver.caltech.edu/CaltechAUTHORS:20201218-095602925

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Abstract

Analysis of E.coli chromosomes isolated under conditions similar to those used for isolation of eukaryotic chromatin has shown that: 1) The proteins of highly purified E.coli deoxyribonucleoprotein are mainly in addition to RNA polymerase two specific histone-like proteins of apparent molecular weight of 17,000 and 9,000 (proteins 1 and 2, respectively). 2) Proteins 1 and 2 occur in approximately equal molar amounts in the isolated E.coli chromosome, and their relative content corresponds to one molecule of protein 1 plus one molecule of protein 2 per 150–200 base pairs of DNA. 3) There are no long stretches of naked DNA in the purified E.coli deoxyribonucleoprotein suggesting a fairly uniform distribution of the proteins 1 and 2 along DNA. 4; The protein 2 is apparently identical to the DNA-binding protein HU which was isolated previously /1/ from extracts of E.coli cells. 5) Digestion of the isolated E.coli chromosomes with staphylococcal nuclease proceeds through discrete deoxyribonucleoprotein intermediates (in particular, at ∼120 base pairs) which contain both proteins 1 and 2. However, since no repeating multimer structure was observed so far in nuclease digests of the E.coli chromosome, it seems premature to draw definite conclusions about possible similarities between the nucleosomal organization of the eukaryotic chromatin and the E.coli chromatin structure.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/4.8.2725DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC342604/PubMed CentralArticle
ORCID:
AuthorORCID
Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1977 Information Retrieval Limited. Received 9 May 1977. We thank M.I. Shifman for his excellent assistance in some of the experiments, V.V.Shmatchenko for advice on acetic acid-urea gel electrophoresis and Dr. R. Hoffman (Harvard Medical School) for a critical reading of the manuscript.
Issue or Number:8
PubMed Central ID:PMC342604
Record Number:CaltechAUTHORS:20201218-095602925
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201218-095602925
Official Citation:A. J. Varshavsky, S. A. Nedospasov, V. V. Bakayev, T. G. Bakayeva, G. P. Georgiev, Histone-like proteins in the purified Escherichia coli deoxyribonucleoprotein, Nucleic Acids Research, Volume 4, Issue 8, 1 August 1977, Pages 2725–2746, https://doi.org/10.1093/nar/4.8.2725
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107197
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Dec 2020 23:40
Last Modified:18 Dec 2020 23:40

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