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Compact form of SV40 viral minichromosome is resistant to nuclease: possible implications for chromatin structure

Varshavsky, A. J. and Nedospasov, S. A. and Schmatchenko, V. V. and Bakayev, V. V. and Chumackov, P. M. and Georgiev, G. P. (1977) Compact form of SV40 viral minichromosome is resistant to nuclease: possible implications for chromatin structure. Nucleic Acids Research, 4 (10). pp. 3303-3325. ISSN 0305-1048. PMCID PMC342655. https://resolver.caltech.edu/CaltechAUTHORS:20201218-095603050

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Abstract

We report two new findings bearing on the “supranucleosomal” level of the structure of the Simian Virus 40 mini-chromosome. I) Isolated SV40 minichromosone which contains all five histones including HI /I exists in solutionunder approximately physiological ionic conditions as a compact roughly spherical particle∼300 Å in diameter which is capable of fitting within the virus capsid. In spite of such a compact conformation of the minichromosome individual nucleosomes can be readily visualized within the particle. Compact state of SV40 minichromosome depends on both the presence of histone HI and maintenance of approximately physiologicalionic strength of solution (μ≈0.15). Removal of HI results in a conversion of the compact minichromosome into an extended (circular beaded) structure. 2) The compact form of the SV40 minichromosome in contrast to its circular beaded form is virtually completely resistant to staphylococcal nuclease, strongly suggesting that in particular nucleasesensitive, parts of the internucleosomal DNA regions are not exposed on the outside of the compact SV40 minichromosome. On the other hand, DNase I which is known to attack both inter- and intranucleosomal DNA in the chromatin /2, 3/ readily digests the compact form of the SV40 minichromosome.Possible models of the compact minichromosome and implications for higher order structures of the cellular chromatin are discussed.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/4.10.3303DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC342655/PubMed CentralArticle
ORCID:
AuthorORCID
Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1997 Information Retrieval Limited. Received 13 June 1977.
Issue or Number:10
PubMed Central ID:PMC342655
Record Number:CaltechAUTHORS:20201218-095603050
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20201218-095603050
Official Citation:A. J. Varshavsky, S. A. Nedospasov, V. V. Schmatchenko, V. V. Bakayev, P. M. Chumackov, G. P. Georgiev, Compact form of SV40 viral minichromosome is resistant to nuclease: possible implications for chromatin structure, Nucleic Acids Research, Volume 4, Issue 10, 1 October 1977, Pages 3303–3325, https://doi.org/10.1093/nar/4.10.3303
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107198
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Dec 2020 23:42
Last Modified:18 Dec 2020 23:42

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