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Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma

Du, Jiajun and Su, Yapeng and Heath, James Richard and Wei, Lu (2020) Raman-guided subcellular pharmaco-metabolomics for metastatic melanoma. In: 260th ACS National Meeting & Exposition, 23-27 August 2020, San Francisco, CA.

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We utilized Raman spectro-microscopy to non-invasively probe metabomics within single live cells, aiming to identify druggable metabolic susceptibilities from a series of patient-derived BRAF mutant melanoma cell lines. Each cell line represents a phenotype with different characteristic level of de-differentiation and BRAFi (BRAF inhibitor) resistance. First, with single-cell Raman spectroscopy and stimulated Raman scattering (SRS) microscopy, followed by transcriptomics anal., we identified lipid processes as major metabolic functional difference between different phenotypes. We then utilized hyperspectral-SRS imaging on intracellular single organelles to identify a previously unknown susceptibility of lipid desatn. within de-differentiated cell lines. Drugging this target leads to cellular apoptosis accompanied by phase sepd. intracellular domains. The integration of subcellular Raman spectro-microscopy with lipidomics and transcriptomics suggests highly heterogenous metabolic responses and possible lipid regulatory mechanisms underlying this pharmacol. treatment. Our method should provide a general approach in spatially-resolved single cell metabolomics studies.

Item Type:Conference or Workshop Item (Paper)
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URLURL TypeDescription Paper ItemJournal Article
Su, Yapeng0000-0002-6305-8467
Heath, James Richard0000-0001-5356-4385
Wei, Lu0000-0001-9170-2283
Additional Information:© 2020 American Chemical Society.
Record Number:CaltechAUTHORS:20201221-101511711
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107235
Deposited By: Tony Diaz
Deposited On:21 Dec 2020 19:33
Last Modified:21 Dec 2020 19:33

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