A Caltech Library Service

miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1

Kato, Mitsuo and Abdollahi, Maryam and Tunduguru, Ragadeepthi and Tsark, Walter and Chen, Zhuo and Wu, Xiwei and Wang, Jinhui and Chen, Zhen Bouman and Lin, Feng-Mao and Lanting, Linda and Wang, Mei and Huss, Janice and Fueger, Patrick T. and Chan, David and Natarajan, Rama (2021) miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Communications Biology, 4 . Art. No. 30. ISSN 2399-3642. PMCID PMC7782535; PMC7862629.

PDF - Published Version
Creative Commons Attribution.

PDF - Erratum
Creative Commons Attribution.

[img] PDF - Supplemental Material
Creative Commons Attribution.

[img] PDF (Description of Additional Supplementary Files) - Supplemental Material
Creative Commons Attribution.

[img] MS Excel (Supplementary Data 1) - Supplemental Material
Creative Commons Attribution.

[img] MS Excel (Supplementary Data 2) - Supplemental Material
Creative Commons Attribution.


Use this Persistent URL to link to this item:


Diabetic kidney disease (DKD) is a major complication of diabetes. Expression of members of the microRNA (miRNA) miR-379 cluster is increased in DKD. miR-379, the most upstream 5′-miRNA in the cluster, functions in endoplasmic reticulum (ER) stress by targeting EDEM3. However, the in vivo functions of miR-379 remain unclear. We created miR-379 knockout (KO) mice using CRISPR-Cas9 nickase and dual guide RNA technique and characterized their phenotype in diabetes. We screened for miR-379 targets in renal mesangial cells from WT vs. miR-379KO mice using AGO2-immunopreciptation and CLASH (cross-linking, ligation, sequencing hybrids) and identified the redox protein thioredoxin and mitochondrial fission-1 protein. miR-379KO mice were protected from features of DKD as well as body weight loss associated with mitochondrial dysfunction, ER- and oxidative stress. These results reveal a role for miR-379 in DKD and metabolic processes via reducing adaptive mitophagy. Strategies targeting miR-379 could offer therapeutic options for DKD.

Item Type:Article
Related URLs:
URLURL TypeDescription Correction CentralArticle CentralPublisher Correction
Kato, Mitsuo0000-0002-1783-1084
Abdollahi, Maryam0000-0002-1989-6068
Tunduguru, Ragadeepthi/0000-0001-7075-5511
Chen, Zhuo0000-0002-3522-9660
Wu, Xiwei0000-0002-7071-1671
Chen, Zhen Bouman0000-0002-3291-1090
Lin, Feng-Mao0000-0003-4042-0096
Fueger, Patrick T.0000-0003-0602-6458
Chan, David0000-0002-0191-2154
Natarajan, Rama0000-0003-4494-1788
Additional Information:© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit Received 27 May 2020; Accepted 23 November 2020; Published 04 January 2021. These studies were supported by grants from the National Institutes of Health (NIH), R01 DK081705, R01 DK058191, and R01 HL106089 (to R.N.), the Wanek Family Project for the Cure of Type 1 diabetes at City of Hope (to R.N. and M.K.), R01HL145170 (to Z.B.C.), the American Diabetes Association ADA-1-18-IBS-103 (to J.H.) and a Postdoctoral fellowship from the Larry L. Hillblom Foundation (to R.T.). We are grateful to members of the Natarajan laboratory for helpful discussions. Research reported in this publication included work performed in the following Cores: Pathology Research Services, Integrative Genomics and Bioinformatics, and Comprehensive Metabolic Phenotyping, supported by the National Cancer Institute of the NIH under award number P30CA33572, as well as the Animal Resource Center, Transgenic/Knockout Animal and Electron Microscopy (EM/AFM) Cores at City of Hope. We are grateful to Dr. Zhuo Li and Ricardo Zerda Noriega of the EM/AFM Core at City of Hope for their help with preparation of samples for TEM and the imaging. Data availability: All sequencing datasets generated in this study have been deposited into GEO with GEO #s GSE142596, GSE142597, and GSE142598. The source data underlying the graphs in figures are provided in Supplementary Data 1 and 2. Full gel is shown in Supplementary Information. All relevant data are available from the authors upon request. These authors contributed equally: Mitsuo Kato, Maryam Abdollahi. Author Contributions: M.K., M.A., and R.N. designed research. M.K., M.A., M.W., R.T., and L.L. performed research. M.K., M.A., Z.C., R.T, and M.W. analyzed the data. W.T. produced the miR-379KO mice. F.-M.L., M.K., Z.B.C., and Z.C. analyzed RNA-seq and hybrid-seq results. D.C. helped with the mitochondria function experiments, J.H. with the Seahorse experiments, and P.T.F. with the mouse metabolic phenotyping. M.K., M.A., and R.N. wrote and edited the manuscript. All authors have read and discussed the manuscript. The authors declare no competing interests.
Errata:Kato, M., Abdollahi, M., Tunduguru, R. et al. Publisher Correction: miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Commun Biol 4, 175 (2021).
Funding AgencyGrant Number
NIHR01 DK081705
NIHR01 DK058191
NIHR01 HL106089
American Diabetes AssociationADA-1-18-IBS-103
Larry L. Hillblom FoundationUNSPECIFIED
Subject Keywords:End-stage renal disease; Genetic engineering
PubMed Central ID:PMC7782535; PMC7862629
Record Number:CaltechAUTHORS:20210107-094647288
Persistent URL:
Official Citation:Kato, M., Abdollahi, M., Tunduguru, R. et al. miR-379 deletion ameliorates features of diabetic kidney disease by enhancing adaptive mitophagy via FIS1. Commun Biol 4, 30 (2021).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107356
Deposited By: Tony Diaz
Deposited On:08 Jan 2021 18:55
Last Modified:18 Feb 2021 17:47

Repository Staff Only: item control page