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Imaging the Beating Heart with Macroscopic Phase Stamping

Trivedi, Vikas and Madaan, Sara and Holland, Daniel B. and Trinh, Le A. and Fraser, Scott E. and Truong, Thai V. (2020) Imaging the Beating Heart with Macroscopic Phase Stamping. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20210111-160848952

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Abstract

We present a novel approach for imaging the beating embryonic heart, based on combining two independent imaging channels to capture the full spatio-temporal information of the moving 3D structure. High-resolution, optically-sectioned image recording is accompanied by simultaneous acquisition of low-resolution, whole-heart recording, allowing the latter to be used in post-acquisition processing to determine the macroscopic spatio-temporal phase of the heart beating cycle. Once determined, or 'stamped', the phase information common to both imaging channels is used to reconstruct the 3D beating heart. We demonstrated our approach in imaging the beating heart of the zebrafish embryo, capturing the entire heart over its full beating cycle, and characterizing cellular dynamic behavior with sub-cellular resolution.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
http://arxiv.org/abs/2011.01688arXivDiscussion Paper
ORCID:
AuthorORCID
Trivedi, Vikas0000-0003-0953-0553
Holland, Daniel B.0000-0002-3404-4939
Fraser, Scott E.0000-0002-5377-0223
Additional Information:Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). We thank Leigh Ann Fletcher and Lorna McFarlane for fish care. This work was funded by the Rosen Center for Bioengineering (Caltech), a Center of Excellence in Genomic Science grant (NIH/NHGRI P50HR004071), a FaceBase grant (NIH Grant U01DE020063) and the Translational Imaging Center at USC.
Group:Rosen Bioengineering Center
Funders:
Funding AgencyGrant Number
Donna and Benjamin M. Rosen Bioengineering CenterUNSPECIFIED
NIHP50HR004071
NIHU01DE020063
University of Southern CaliforniaUNSPECIFIED
Record Number:CaltechAUTHORS:20210111-160848952
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210111-160848952
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107407
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:12 Jan 2021 16:00
Last Modified:12 Jan 2021 16:00

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