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Imaging the Beating Heart with Macroscopic Phase Stamping

Trivedi, Vikas and Madaan, Sara and Holland, Daniel B. and Trinh, Le A. and Fraser, Scott E. and Truong, Thai V. (2020) Imaging the Beating Heart with Macroscopic Phase Stamping. . (Unpublished)

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We present a novel approach for imaging the beating embryonic heart, based on combining two independent imaging channels to capture the full spatio-temporal information of the moving 3D structure. High-resolution, optically-sectioned image recording is accompanied by simultaneous acquisition of low-resolution, whole-heart recording, allowing the latter to be used in post-acquisition processing to determine the macroscopic spatio-temporal phase of the heart beating cycle. Once determined, or 'stamped', the phase information common to both imaging channels is used to reconstruct the 3D beating heart. We demonstrated our approach in imaging the beating heart of the zebrafish embryo, capturing the entire heart over its full beating cycle, and characterizing cellular dynamic behavior with sub-cellular resolution.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Trivedi, Vikas0000-0003-0953-0553
Holland, Daniel B.0000-0002-3404-4939
Fraser, Scott E.0000-0002-5377-0223
Additional Information:Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). We thank Leigh Ann Fletcher and Lorna McFarlane for fish care. This work was funded by the Rosen Center for Bioengineering (Caltech), a Center of Excellence in Genomic Science grant (NIH/NHGRI P50HR004071), a FaceBase grant (NIH Grant U01DE020063) and the Translational Imaging Center at USC.
Group:Rosen Bioengineering Center
Funding AgencyGrant Number
Donna and Benjamin M. Rosen Bioengineering CenterUNSPECIFIED
University of Southern CaliforniaUNSPECIFIED
Record Number:CaltechAUTHORS:20210111-160848952
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107407
Deposited By: George Porter
Deposited On:12 Jan 2021 16:00
Last Modified:12 Jan 2021 16:00

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