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Nanoengineered shear-thinning and bioprintable hydrogel as a versatile platform for biomedical applications

Zandi, Nooshin and Sani, Ehsan Shirzaei and Mostafavi, Ebrahim and Ibrahim, Dina M. and Saleh, Bahram and Shokrgozar, Mohammad Ali and Tamjid, Elnaz and Weiss, Paul S. and Simchi, Abdolreza and Annabi, Nasim (2021) Nanoengineered shear-thinning and bioprintable hydrogel as a versatile platform for biomedical applications. Biomaterials, 267 . Art. No. 120476. ISSN 0142-9612. https://resolver.caltech.edu/CaltechAUTHORS:20210112-144610536

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Abstract

The development of bioinks based on shear-thinning and self-healing hydrogels has recently attracted significant attention for constructing complex three-dimensional physiological microenvironments. For extrusion-based bioprinting, it is challenging to provide high structural reliability and resolution of printed structures while protecting cells from shear forces during printing. Herein, we present shear-thinning and printable hydrogels based on silicate nanomaterials, laponite (LA), and glycosaminoglycan nanoparticles (GAGNPs) for bioprinting applications. Nanocomposite hydrogels (GLgels) were rapidly formed within seconds due to the interactions between the negatively charged groups of GAGNPs and the edges of LA. The shear-thinning behavior of the hydrogel protected encapsulated cells from aggressive shear stresses during bioprinting. The bioinks could be printed straightforwardly into shape-persistent and free-standing structures with high aspect ratios. Rheological studies demonstrated fast recovery of GLgels over multiple strain cycles. In vitro studies confirmed the ability of GLgels to support cell growth, proliferation, and spreading. In vitro osteogenic differentiation of pre-osteoblasts murine bone marrow stromal cells encapsulated inside the GLgels was also demonstrated through evaluation of ALP activity and calcium deposition. The subcutaneous implantation of the GLgel in rats confirmed its in vivo biocompatibility and biodegradability. The engineered shear-thinning hydrogel with osteoinductive characteristics can be used as a new bioink for 3D printing of constructs for bone tissue engineering applications.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.biomaterials.2020.120476DOIArticle
Additional Information:Published by Elsevier. Received 2 March 2020, Revised 13 October 2020, Accepted 18 October 2020, Available online 19 October 2020. NA acknowledges the support from American Heart Association (AHA, 16SDG31280010), and National Institutes of Health (NIH) (R01EB023052; R01HL140618). AS wishes to thank the financial support of Iran National Science Foundation (INSF No. 95-S-48740) and Sharif University of Technology (Grant No. QA970816). CRediT authorship contribution statement. Nooshin Zandi: Conceptualization, Methodology, Formal analysis, Investigation, Data curation, Writing - original draft, Writing - review & editing, Project administration. Ehsan Shirzaei Sani: Investigation, Writing - review & editing, Formal analysis. Ebrahim Mostafavi: Investigation, Writing - review & editing, Formal analysis. Dina M. Ibrahim: Investigation, Formal analysis. Bahram Saleh: Investigation. Mohammad Ali Shokrgozar: Supervision. Elnaz Tamjid: Supervision. Paul S. Weiss: Writing - review & editing. Abdolreza Simchi: Writing - review & editing, Supervision, Resources, Funding acquisition. Nasim Annabi: Conceptualization, Supervision, Writing - review & editing, Resources, Funding acquisition, Validation. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Funders:
Funding AgencyGrant Number
American Heart Association16SDG31280010
NIHR01EB023052
NIHR01HL140618
Iran National Science Foundation95-S-48740
Sharif University of TechnologyQA970816
Subject Keywords:3D bioprinting; Biomimetic proteoglycan; Osteoinductive bioink; Nanocomposite; Laponite
Record Number:CaltechAUTHORS:20210112-144610536
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210112-144610536
Official Citation:Nooshin Zandi, Ehsan Shirzaei Sani, Ebrahim Mostafavi, Dina M. Ibrahim, Bahram Saleh, Mohammad Ali Shokrgozar, Elnaz Tamjid, Paul S. Weiss, Abdolreza Simchi, Nasim Annabi, Nanoengineered shear-thinning and bioprintable hydrogel as a versatile platform for biomedical applications, Biomaterials, Volume 267, 2021, 120476, ISSN 0142-9612, https://doi.org/10.1016/j.biomaterials.2020.120476.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107440
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:13 Jan 2021 15:29
Last Modified:13 Jan 2021 15:29

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