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Heteroduplex analysis of the sequence relations between the RNAs of mink cell focus-inducing and murine leukemia viruses

Chien, Yueh-Hsiu and Verma, Inder M. and Shih, Thomas Y. and Scolnick, Edward M. and Davidson, Norman (1978) Heteroduplex analysis of the sequence relations between the RNAs of mink cell focus-inducing and murine leukemia viruses. Journal of Virology, 28 (1). pp. 352-360. ISSN 0022-538X.

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The sequence relationships betwen AKR ecotropic virus and an AKR-derived "mink cell focus-inducing" (MCF) isolate (AKR MCF 247), between Moloney murine leukemia virus (M-MLV) and an M-MLV MCF isolate (M-MLV83), and between AKR and M-MLV were studied by electron microscopic heteroduplex analysis. The MCF-specific sequences were found to map from 1.95 kilobases (kb) to 2.75 kb (+/- 0.15 kb) from the 3' end of the RNAs for both MCF isolates. The major sequence nonhomology regions between AKR and M-MLV lie between 0.9 and 3.5 kb from the 3' end. However, the AKR and M-MLV sequences immediately adjacent to the 1.95- and 2.75-kb junctions with MCF-specific sequences are relatively similar in AKR and M-MLV. Our results suggest that the env gene of MLVs maps from 1 kb to 3 kb from the 3' end of the genomic RNA and that the carboxyl end of the glycoprotein of each MCF strain is similar (or identical) to that of its ecotropic parent.

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Additional Information:Copyright © 1978 by the American Society for Microbiology. Received for publication 1 May 1978. We thank Fred Jensen of Scripps Clinic and Research Foundation for a gift of AKR MCF 247 virus and for advice and assistance, and Billie Green and Marianne McKenett for technical assistance. Y.-H.C. has been supported in part by postdoctoral fellowship F32CA05099 from the National Institutes of Health. This research was supported by contract NO1 CP 43306 with the Virus Cancer Program of the National Cancer Institute (N.D.) and by Public Health Service grants CA 16561 and 21408 from the National Cancer Institute. Contribution no. 5769 from the Department of Chemistry, California Institute of Technology.
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Deposited On:05 Jun 2008
Last Modified:03 Oct 2019 00:12

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