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The yeast ubiquitin genes: a family of natural gene fusions

Özkaynak, Engin and Finley, Daniel and Solomon, Mark J. and Varshavsky, Alexander (1987) The yeast ubiquitin genes: a family of natural gene fusions. EMBO Journal, 6 (5). pp. 1429-1439. ISSN 0261-4189. PMCID PMC553949. https://resolver.caltech.edu/CaltechAUTHORS:20210119-114432603

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Abstract

Ubiquitin is a 76‐residue protein highly conserved among eukaryotes. Conjugation of ubiquitin to intracellular proteins mediates their selective degradation in vivo. We describe a family of four ubiquitin‐coding loci in the yeast Saccharomyces cerevisiae. UB11, UB12 and UB13 encode hybrid proteins in which ubiquitin is fused to unrelated (‘tail’) amino acid sequences. The ubiquitin coding elements of UB11 and UB12 are interrupted at identical positions by non‐homologous introns. UB11 and UB12 encode identical 52‐residue tails, whereas UB13 encodes a different 76‐residue tail. The tail amino acid sequences are highly conserved between yeast and mammals. Each tail contains a putative metal‐binding, nucleic acid‐binding domain of the form Cys‐X2‐4‐Cys‐X2‐15‐Cys‐X2‐4‐Cys, suggesting that these proteins may function by binding to DNA. The fourth gene, UB14, encodes a polyubiquitin precursor protein containing five ubiquitin repeats in a head‐to‐tail, spacerless arrangement. All four ubiquitin genes are expressed in exponentially growing cells, while in stationary‐phase cells the expression of UB11 and UB12 is repressed. The UB14 gene, which is strongly inducible by starvation, high temperatures and other stresses, contains in its upstream region strong homologies to the consensus ‘heat shock box’ nucleotide sequence. Elsewhere we show that the essential function of the UB14 gene is to provide ubiquitin to cells under stress.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/j.1460-2075.1987.tb02384.xDOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC553949/PubMed CentralArticle
ORCID:
AuthorORCID
Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© 1987 IRL Press Limited. Received on January 26, 1987. We thank Peter Novick for the Ycp50-based DNA library, Gordon Carmichael for advice on the electrophoresis of RNA, and Barbara Doran for secretarial assistance. E.Ö. is especially grateful to Ulya Özkaynak for her encouragement and help during this work. Supported by a grant to A.V. from the National Institute of General Medical Sciences (GM31530). E.Ö. was supported by a fellowship from the Charles A. King Trust and the Medical Foundation.
Funders:
Funding AgencyGrant Number
NIHGM31530
Charles A. King TrustUNSPECIFIED
Medical FoundationUNSPECIFIED
Subject Keywords:ubiquitin/multigene family/protein turnover/TFIIIA motif/yeast
Issue or Number:5
PubMed Central ID:PMC553949
Record Number:CaltechAUTHORS:20210119-114432603
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210119-114432603
Official Citation:Ozkaynak, E., Finley, D., Solomon, M.J. and Varshavsky, A. (1987), The yeast ubiquitin genes: a family of natural gene fusions.. The EMBO Journal, 6: 1429-1439. https://doi.org/10.1002/j.1460-2075.1987.tb02384.x
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107538
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jan 2021 20:45
Last Modified:19 Jan 2021 20:45

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