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Evolution of the cytochrome-bd type oxygen reductase superfamily and the function of cydAA in Archaea

Murali, Ranjani and Gennis, Robert B. and Hemp, James (2021) Evolution of the cytochrome-bd type oxygen reductase superfamily and the function of cydAA in Archaea. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20210119-125421091

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Abstract

Cytochrome bd-type oxygen reductases (cytbd) belong to one of three enzyme superfamilies that catalyze oxygen reduction to water. They are widely distributed in Bacteria and Archaea, but the full extent of their biochemical diversity is unknown. Here we used phylogenomics to identify 3 families and several subfamilies within the cytbd superfamily. The core architecture shared by all members of the superfamily consists of four transmembrane helices that bind two active site hemes, which are responsible for oxygen reduction. While previously characterized cytochrome bd-type oxygen reductases use quinol as an electron donor to reduce oxygen, sequence analysis shows that only one of the identified families has a conserved quinol binding site. The other families are missing this feature, suggesting that they use an alternative electron donor. Multiple gene duplication events were identified within the superfamily, resulting in significant evolutionary and structural diversity. The CydAA’ cytbd, found exclusively in Archaea, is formed by the co-association of two superfamily paralogs. We heterologously expressed CydAA’ from Caldivirga maquilingensis and demonstrated that it performs oxygen reduction with quinol as an electron donor. Strikingly, CydAA’ is the first isoform of cytbd containing only b-type hemes shown to be active when isolated, demonstrating that oxygen reductase activity in this superfamily is not dependent on heme d.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2021.01.16.426971DOIDiscussion Paper
ORCID:
AuthorORCID
Hemp, James0000-0001-7193-0553
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This version posted January 17, 2021. The authors have declared no competing interest.
Record Number:CaltechAUTHORS:20210119-125421091
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210119-125421091
Official Citation:Evolution of the cytochrome-bd type oxygen reductase superfamily and the function of cydAA’ in Archaea. Ranjani Murali, Robert B. Gennis, James Hemp. bioRxiv 2021.01.16.426971; doi: https://doi.org/10.1101/2021.01.16.426971
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107544
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Jan 2021 21:38
Last Modified:19 Jan 2021 21:38

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