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A technique of low-pH gel electrophoresis of chromosomal proteins which does not require preliminary removal of DNA

Shmatchenko, V. V. and Varshavsky, A. J. (1978) A technique of low-pH gel electrophoresis of chromosomal proteins which does not require preliminary removal of DNA. Analytical Biochemistry, 85 (1). pp. 42-46. ISSN 0003-2697. https://resolver.caltech.edu/CaltechAUTHORS:20210119-135308547

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Abstract

Fractionation of chromosomal proteins, in particular, of histones, by acetic acid-urea polyacrylamide gel electrophoresis usually requires preliminary removal of DNA from deoxyribonucleoprotein samples to obtain good separation of proteins. We have found that this difficulty can be overcome by addition of cetyltrimethylammonium bromide (CTAB) to the gel and electrode buffers. Since CTAB can readily diffuse into polyacrylamide gels two-dimensional fractionation becomes possible; that is, deoxyribonucleoprotein particles are fractionated in the first dimension followed by immersion of a gel in a CTAB solution and then low-pH gel electrophoresis of proteins in the second dimension.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/0003-2697(78)90271-3DOIArticle
ORCID:
AuthorORCID
Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1978 Academic Press. Received 1 June 1977, Accepted 8 September 1977. We thank Dr. V. A. Berdnikov for advice on the use of slab gels and Professor G. P. Georgiev and Dr. V. V. Bakayev for helpful discussions.
Issue or Number:1
Record Number:CaltechAUTHORS:20210119-135308547
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210119-135308547
Official Citation:V.V. Shmatchenko, A.J. Varshavsky, A technique of low-pH gel electrophoresis of chromosomal proteins which does not require preliminary removal of DNA, Analytical Biochemistry, Volume 85, Issue 1, 1978, Pages 42-46, ISSN 0003-2697, https://doi.org/10.1016/0003-2697(78)90271-3.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107551
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jan 2021 22:15
Last Modified:19 Jan 2021 22:15

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