CaltechAUTHORS
  A Caltech Library Service

SV40 viral minichromosome: preferential exposure of the origin of replication as probed by restriction endonucleases

Varshavsky, A. J. and Sundin, O. H. and Bohn, M. J. (1978) SV40 viral minichromosome: preferential exposure of the origin of replication as probed by restriction endonucleases. Nucleic Acids Research, 5 (10). pp. 3469-3478. ISSN 0305-1048. PMCID PMC342688. https://resolver.caltech.edu/CaltechAUTHORS:20210119-135308672

[img] PDF - Published Version
See Usage Policy.

777Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20210119-135308672

Abstract

Isolated SV40 minichromosomes [1–3] were treated with different single-cut restriction endonucleases to probe the arrangement of nucleosomes in relation to the SV40 DNA sequence. While Eco RI and Bam HI each cut 22–27% of the SV40 minichromosomes under limit-digest conditions, Bgl I, which cuts SV40 DNA at or very near the origin of replication [4,5], cleaves 90–95% of the minichromosomes in a preparation. Similar results were obtained with minichromosomes which had been fixed with formaldehyde before endonuclease treatment. One possible interpretation of these findings is that the arrangement of nucleosomes in the compact SV40 minichromosomes is nonrandom at least with regard to sequences near the origin of DNA replication.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/5.10.3469DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC342688/PubMed CentralArticle
ORCID:
AuthorORCID
Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1978 Information Retrieval Limited. Received 10 August 1978. We thank Bruce Yazejian and Frederic Boyce for their excellent technical assistance. One of us (A.J.V.) is deeply indebted to many colleagues both at and outside M.I.T. for their encouragement, support and generous sharing of equipment and facilities during the course of this work.
Issue or Number:10
PubMed Central ID:PMC342688
Record Number:CaltechAUTHORS:20210119-135308672
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210119-135308672
Official Citation:A.J. Varshavsky, O.H. Sundin, M.J. Bohn, SV40 viral minichromosome: preferential exposure of the origin of replication as probed by restriction endonucleases, Nucleic Acids Research, Volume 5, Issue 10, 1 October 1978, Pages 3469–3478, https://doi.org/10.1093/nar/5.10.3469
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107552
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:19 Jan 2021 22:10
Last Modified:23 Jan 2021 00:28

Repository Staff Only: item control page