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Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli. I. Location of "Late" MS2-Specific Ribonucleic Acid Synthesis

Haywood, Anne M. and Cramer, Jane Harris and Shoemaker, Nadja Lancaster (1969) Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli. I. Location of "Late" MS2-Specific Ribonucleic Acid Synthesis. Journal of Virology, 4 (4). pp. 364-371. ISSN 0022-538X. PMCID PMC375883. https://resolver.caltech.edu/CaltechAUTHORS:HAYjvir69

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Abstract

When actinomycin-treated, MS2-infected Escherichia coli are labeled during a brief period later than 16 min after infection, the newly synthesized MS2 ribonucleic acid (RNA) appears first in the 30,000 x g sediment, probably bound to fragments of bacterial membranes, since the radioactivity can be released from the sediment with deoxycholate or urea. With longer labeling times, radioactivity also appears in the 30,000 x g supernatant fluid. While on the membrane, the RNA is organized into particles with sedimentation coefficients of 40, 32, and 27S in the presence of low Mg2+. In the presence of high Mg+, MS2-specific RNA is found in polyribosomes. These data are interpreted to mean that MS2-specific RNA is synthesized and organized into larger structures on membrane. More than 8 min of labeling is required before radioactivity is found in the 81S virion which appears in the supernatant fluid.


Item Type:Article
Related URLs:
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC375883/PubMed CentralArticle
http://jvi.asm.org/cgi/content/abstract/4/4/364OtherUNSPECIFIED
http://jvi.asm.org/cgi/content/abstract/4/4/364OtherUNSPECIFIED
Additional Information:© 1969 American Society for Microbiology. Received for publication 2 June 1969. We are grateful to Karen Williams and Anne Greenwood for technical assistance. A.M.H. acknowledges ideas from conversations with George Guthrie and Robert Haselkorn that led to an interest in association of MS2-specific proteins and subsequently MS2-specific RNA with "membrane." We are greatly indebted to James and Ellen Strauss for editorial help. This research was supported by Public Health Service grants 1 R01 A106549 and 1 R01 A107788 from the National Institute of Allergy and Infectious Diseases. J.H.C. was supported by National Institutes of Health predoctoral training grant 5 TI GM 724.
Funders:
Funding AgencyGrant Number
NIH1 R01 A106549
NIH1 R01 A107788
NIH Predoctoral Fellowship5 TI GM 724
Issue or Number:4
PubMed Central ID:PMC375883
Record Number:CaltechAUTHORS:HAYjvir69
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:HAYjvir69
Official Citation:Host-Virus Interaction in Ribonucleic Acid Bacteriophage-Infected Escherichia coli Anne M. Haywood, Jane Harris Cramer, Nadja Lancaster Shoemaker Journal of Virology Oct 1969, 4 (4) 364-371
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:10760
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:06 Jun 2008
Last Modified:03 Oct 2019 00:12

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