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Transcriptome-Scale Super-Resolved Imaging in Tissues by RNA SeqFISH

Cai, L. (2020) Transcriptome-Scale Super-Resolved Imaging in Tissues by RNA SeqFISH. European Journal of Human Genetics, 28 (S1). p. 10. ISSN 1018-4813. doi:10.1038/s41431-020-00740-6.

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Imaging the transcriptome in situ with high accuracy has been a major challenge in single cell biology, particularly hindered by the limits of optical resolution and the density of transcripts in single cells. We developed seqFISH+, that can image the mRNAs for 10,000 genes in single cells with high accuracy and sub-diffraction-limit resolution, in the mouse brain cortex, subventricular zone, and the olfactory bulb, using a standard confocal microscope. The transcriptome level profiling of seqFISH+ allows unbiased identification of cell classes and their spatial organization in tissues. In addition, seqFISH+ reveals subcellular mRNA localization patterns in cells and ligand-receptor pairs across neighboring cells. This technology demonstrates the ability to generate spatial cell atlases and to perform discovery-driven studies of biological processes in situ.

Item Type:Article
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URLURL TypeDescription ReadCube access - Abstracts ItemJournal Article
Cai, L.0000-0002-7154-5361
Additional Information:© 2020 Nature Publishing Group. Published 01 December 2020.
Issue or Number:S1
Record Number:CaltechAUTHORS:20210120-154339392
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Official Citation:Abstracts from the 53rd European Society of Human Genetics (ESHG) Conference: Oral Presentations. Eur J Hum Genet 28, 1–140 (2020).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107608
Deposited By: Tony Diaz
Deposited On:21 Jan 2021 00:21
Last Modified:16 Nov 2021 19:04

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