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Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but strongly depends on DNA fragment size

Swerdlow, Paul S. and Varshavsky, Alexander (1983) Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but strongly depends on DNA fragment size. Nucleic Acids Research, 11 (2). pp. 387-401. ISSN 0305-1048. PMCID PMC325721. https://resolver.caltech.edu/CaltechAUTHORS:20210122-150600293

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Abstract

We have used a two-dimensional (deoxyribonucleoprotein →DNA) electrophoretic binding assay to study the interaction of the purified high mobility group protein HMG17 with isolated HeLa mononucleosomes as a function of their DNA fragment size and the presence of ubiquitin-H2A semihistone. No significant differences between affinities of HMG17 for ubiquitinated and non-ubiquitinated core mononucleosomes were observed. In striking contrast, the apparent affinity of HMG17 for a mononucleosome increases more than 100-fold upon an increase of the length of the mononucleosomal DNA fragment by as few as 3 to 5 bp over the core DNA length (∽146 bp). We suggest that the magnitude of this effect is sufficient to explain the preferential binding of HMG17 in vitro to mononucleosomes derived from actively transcribed genes.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/nar/11.2.387DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC325721PubMed CentralArticle
ORCID:
AuthorORCID
Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© IRL Press Limited 1983. Received 29 September 1982; Revised 9 December 1982; Accepted 23 December 1982. We are greatly indebted to Louis Levinger, as well as James Barsoum and Daniel Finley for the many helpful discussions and advice. This work was supported by grants to A.V. from the National Cancer Institute and the National Institute of General Medical Sciences. P.S. was supported by a postdoctoral fellowship from Damon Runyon-Walter Winchel Cancer Fund, and earlier by a training grant (5T32-HL07146) from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
National Cancer InstituteUNSPECIFIED
National Institute of General Medical SciencesUNSPECIFIED
Damon Runyon-Walter Winchell Cancer FundUNSPECIFIED
NIH Predoctoral Fellowship5T32-HL07146
Issue or Number:2
PubMed Central ID:PMC325721
Record Number:CaltechAUTHORS:20210122-150600293
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210122-150600293
Official Citation:Paul S. Swerdlow, Alexander Varshavsky, Affinity of HMG17 for a mononucleosome is not influenced by the presence of ubiquitin-H2A semihistone but strongly depends on DNA fragment size, Nucleic Acids Research, Volume 11, Issue 2, 25 January 1983, Pages 387–401, https://doi.org/10.1093/nar/11.2.387
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107675
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Jan 2021 23:33
Last Modified:22 Jan 2021 23:33

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