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Cellular and SV40 Chromatin: Replication, Segregation, Ubiqiritination, Nuclease-hypersensitive Sites, HMG-containing Nueleosomes, and Heterochromatin-specific Protein

Varshavsky, A. and Levinger, L. and Sundin, O. and Barsoum, J. and Özkaynak, E. and Swerdlow, P. and Finley, D. (1983) Cellular and SV40 Chromatin: Replication, Segregation, Ubiqiritination, Nuclease-hypersensitive Sites, HMG-containing Nueleosomes, and Heterochromatin-specific Protein. Cold Spring Harbor Symposia on Quantitative Biology, 47 . pp. 511-528. ISSN 0091-7451. https://resolver.caltech.edu/CaltechAUTHORS:20210122-150600550

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Abstract

During lytic infection, SV40 DNA exists as a minichromosome within the host nucleus and is replicated in a manner similar to that of the cellular genome. The 5.2-kb circular, double-stranded SV40 DNA directs site-specific synthesis of a single replication “bubble,” which is then enlarged through movement of both replication forks (for review, see DePamphilis and Wassarman 1980). Separate, covalently closed, circular SV40 DNA molecules appear approximately 5 minutes after the forks meet halfway around the genome (Levine et al. 1970). We have recently discovered a new class of SV40 replicative intermediates participating in these latest stages of SV40 DNA replication (Sundin and Varshavsky 1980, 1981). All members of this class are catenated dimers, two circular molecules of SV40 DNA linked topologically by one or more intertwining events. The catenated SV40 DNA dimers comprise three specific families and occur as minichromosomes. They are all rapidly processed in vivo to mature supercoiled SV40 DNA I. We have also found conditions for selective arrest of SV40 replication in vivo at the stage of catenated chromosomes (Sundin and Varshavsky 1981). SV40 is the first example of a circular, double-stranded replicon that uses multiply intertwined catenated dimers as segregation intermediates. It is possible that analogous mechanisms operate during the final stages of replication in a variety of other eukaryotic and prokaryotic replicons. Some of the early studies on singly intertwined catenated dimers in mitochondrial, SV40, and other DNAs are those done by Rush et al. (1970), Jaenisch and Levine (1972), Novick et al. (1973), and Kupersztoch and Helinski (1973). What follows is a condensed account of the "DNA-level" work on multiply intertwined catenated dimers (Sundin and Varshavsky 1980, 1981), as well as our more recent data on the chromatin organization of SV40 chromosomes during the segregation of daughter DNA molecules.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/sqb.1983.047.01.061DOIArticle
ORCID:
AuthorORCID
Varshavsky, A.0000-0002-4011-258X
Additional Information:© 1983 by Cold Spring Harbor Laboratory Press. We are greatly indebted to Douglas Brutlag, Mathew Meselson, Gerald Rubin, and Robert Schimke for the gifts of DNA clones used in this work; to Joseph Bertino for L5178Y-R(C3) cells; to Frederick Boyce for his collaboration in some of the SV40 experiments, and to Aaron Ciechanover, Mark Solomon, and Richard Pan for helpful discussions. This work was supported by grants to A.V. from the National Cancer Institute and the National Institute of General Medical Sciences. L.L. and E.Ö. were supported by postdoctoral fellowships from the Medical Foundation and the Leukemia Society of America, respectively. J.B., D.F., and P.S. were supported by departmental training grants (GM-07287 and 5T32-HL-07146) from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
National Cancer InstituteUNSPECIFIED
National Institute of General Medical SciencesUNSPECIFIED
Medical FoundationUNSPECIFIED
Leukemia Society of AmericaUNSPECIFIED
NIH Predoctoral FellowshipGM-07287
NIH Predoctoral Fellowship5T32-HL-07146
Record Number:CaltechAUTHORS:20210122-150600550
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210122-150600550
Official Citation:Cellular and SV40 Chromatin: Replication, Segregation, Ubiqiritination, Nuclease-hypersensitive Sites, HMG-containing Nueleosomes, and Heterochromatin-specific Protein A. Varshavsky, L. Levinger, O. Sundin, J. Barsoum, E. Özkaynak, P. Swerdlow, and D. Finley Cold Spring Harb Symp Quant Biol 1983 47: 511-528; doi:10.1101/SQB.1983.047.01.061
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107677
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:22 Jan 2021 23:30
Last Modified:22 Jan 2021 23:30

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