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The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme

Goebl, Mark G. and Yochem, John and Jentsch, Stefan and McGrath, John P. and Varshavsky, Alexander and Byers, Breck (1988) The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme. Science, 241 (4871). pp. 1331-1335. ISSN 0036-8075. doi:10.1126/science.2842867. https://resolver.caltech.edu/CaltechAUTHORS:20210129-142403433

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Abstract

Mutants in the gene CDC34 of the yeast Saccharomyces cerevisiae are defective in the transition from G1 to the S phase of the cell cycle. This gene was cloned and shown to encode a 295-residue protein that has substantial sequence similarity to the product of the yeast RAD6 gene. The RAD6 gene is required for a variety of cellular functions including DNA repair and was recently shown to encode a ubiquitin-conjugating enzyme. When produced in Escherichia coli, the CDC34 gene product catalyzed the covalent attachment of ubiquitin to histones H2A and H2B in vitro, demonstrating that the CDC34 protein is another distinct member of the family of ubiquitin-conjugating enzymes. The cell cycle function of CDC34 is thus likely to be mediated by the ubiquitin-conjugating activity of its product.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.2842867DOIArticle
ORCID:
AuthorORCID
Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© 1988 American Association for the Advancement of Science. 9 March 1988; accepted 29 June 1988. We thank L. Hartweli, W. Fangman, A. Hopper, J. Hurley, D. Finley, and B. Bartel for advice during the preparation of the manuscript. We thank C. Mann for the gift of plasmid CMp170 and R. McCarroll for the Southern blot of separated yeast chromosomes. Supported by grants from the National Institute of General Medical Sciences to B.B. (GM18541) and A.V. (GM31530).
Funders:
Funding AgencyGrant Number
NIHGM18541
NIHGM31530
Issue or Number:4871
DOI:10.1126/science.2842867
Record Number:CaltechAUTHORS:20210129-142403433
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210129-142403433
Official Citation:The yeast cell cycle gene CDC34 encodes a ubiquitin-conjugating enzyme BY MG GOEBL, J YOCHEM, S JENTSCH, JP MCGRATH, A VARSHAVSKY, B BYERS Science 09 Sep 1988: Vol. 241, Issue 4871, pp. 1331-1335 DOI: 10.1126/science.2842867
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107825
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:01 Feb 2021 15:16
Last Modified:16 Nov 2021 19:06

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