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Hypersensitivity to heavy water: A new conditional phenotype

Bartel, Bonnie and Varshavsky, Alexander (1988) Hypersensitivity to heavy water: A new conditional phenotype. Cell, 52 (6). pp. 935-941. ISSN 0092-8674. doi:10.1016/0092-8674(88)90435-7.

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Wild-type strains of the yeast S. cerevisiae can grow on media containing 90% D₂O. Using chemical mutagenesis we obtained a number of strains that grow on H₂O-containing media but not on otherwise identical media containing 90% D₂O. The frequency of these D₂O-sensitive (ds) mutants is comparable to the frequency of conventional temperature-sensitive (ts) mutants in the same mutagenized sample, and the ds mutations are distributed over a large number of complementation groups. Furthermore, most ds mutants do not display other conditional phenotypes, such as heat, cold, or osmotic sensitivity. Conversely, of 17 cell division cycle is mutants tested, only 2 are also ds. Thus, the ds technique should be useful for producing conditional mutations in genes that are not amenable to the is and cs approaches, and also for generating alternative conditional (ds) alleles in many other genes. In addition, the ds technique should make it possible to generate conditional (ds) mutants in homeothermic animals, thereby extending the advantages of conditional phenotypes to mammalian and avian genetics.

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Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© 1988 Cell Press. Received January 21, 1988; revised February 24, 1988. We thank Daniel Finley and John McGrath for comments on the manuscript, and Barbara Doran for secretarial assistance. One of us (A. V.) is grateful to Alexandra Hawiger for helpful discussions at the beginning of this work. This work was supported by a grant to A. V. from the National Cancer Institute (CA43309). B. B. was supported by a predoctoral fellowship from the National Science Foundation. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked ‘advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
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NSF Graduate Research FellowshipUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20210129-142403546
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Official Citation:Bonnie Bartel, Alexander Varshavsky, Hypersensitivity to heavy water: A new conditional phenotype, Cell, Volume 52, Issue 6, 1988, Pages 935-941, ISSN 0092-8674,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107826
Deposited By: George Porter
Deposited On:01 Feb 2021 15:20
Last Modified:16 Nov 2021 19:06

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