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Free DNA stretches in histone H1-depleted chromatin and their possible relation to chromomere structure

Varshavsky, A. J. and Georgiev, G. P. (1976) Free DNA stretches in histone H1-depleted chromatin and their possible relation to chromomere structure. Molecular Biology Reports, 3 (1). pp. 27-38. ISSN 0301-4851. https://resolver.caltech.edu/CaltechAUTHORS:20210203-074258460

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Abstract

Oligomers of chromatin subunits (oligonucleosomes) were prepared by a mild digestion of chromatin with staphylococcal nuclease followed by a purification of a high molecular weight material (hexanucleosomes and larger DNP particles) by gel chromatography. The main finding is that a mild removal of histone H1 from the oligonucleosome preparation by treatment with tRNA in the absence of any significant hydrodynamic shearing leads to the formation of free DNA molecules which constitute 5–6% of the total oligonucleosomal DNA. The size of nucleosome-free DNA stretches in H1-depleted hydrodynamically sheared chromatin is about 6000 base pairs and their content is apparently 10–12% of the total DNA. These and related findings are discussed in terms of the previously proposed ‘asymmetric hairpin’ model of DNA packing in chromatin. Different kinds of the asymmetric hairpin are considered and ambiguities in interpretations of experimental data are pointed out.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/bf00357206DOIArticle
ORCID:
AuthorORCID
Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1976 Springer Science and Business Media LLC. Received 15 May 1976.
Subject Keywords:Experimental Data; Molecular Weight; Oligomer; Base Pair; High Molecular Weight
Issue or Number:1
Record Number:CaltechAUTHORS:20210203-074258460
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210203-074258460
Official Citation:Varshavsky, A.J., Georgiev, G.P. Free DNA stretches in histone H1-depleted chromatin and their possible relation to chromomere structure. Molecular Biology Reports 3, 27–38 (1976). https://doi.org/10.1007/BF00357206
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107886
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:03 Feb 2021 16:48
Last Modified:03 Feb 2021 16:48

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