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The N-end rule in bacteria

Tobias, John W. and Shrader, Thomas E. and Rocap, Gabrielle and Varshavsky, Alexander (1991) The N-end rule in bacteria. Science, 254 (5036). pp. 1374-1377. ISSN 0036-8075. doi:10.1126/science.1962196. https://resolver.caltech.edu/CaltechAUTHORS:20210210-151525693

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Abstract

The N-end rule relates the in vivo half-life of a protein to the identity of its amino-terminal residue. Distinct versions of the N-end rule operate in all eukaryotes examined. It is shown that the bacterium Escherichia coli also has the N-end rule pathway. Amino-terminal arginine, lysine, leucine, phenylalanine, tyrosine, and tryptophan confer 2-minute half-lives on a test protein; the other amino-terminal residues confer greater than 10-hour half-lives on the same protein. Amino-terminal arginine and lysine are secondary destabilizing residues in E. coli because their activity depends on their conjugation to the primary destabilizing residues leucine or phenylalanine by leucine, phenylalanine-transfer RNA-protein transferase. The adenosine triphosphate-dependent protease Clp (Ti) is required for the degradation of N-end rule substrates in E. coli.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1126/science.1962196DOIArticle
ORCID:
AuthorORCID
Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© 1991 American Association for the Advancement of Science. 6 June 1991; accepted 9 September 1991. We thank B. Wanner for the BW13711 strain; D. Parcel, R. Sauer, W. Waker, C. Gross, and B. Bachman for other E. coli strains; G. Walker and members of his laboratory for advice; J. Essigman for making PhosphorImager available to us; and B. Doran for secretarial assistance. Supported by NIH grant DK39520 and GM31530 (to A.V.). T.E.S. is a Merck Fellow of the Helen Hay Whitney Foundation.
Funders:
Funding AgencyGrant Number
NIHDK39520
NIHGM31530
Helen Hay Whitney FoundationUNSPECIFIED
Issue or Number:5036
DOI:10.1126/science.1962196
Record Number:CaltechAUTHORS:20210210-151525693
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210210-151525693
Official Citation:The N-end rule in bacteria BY JW TOBIAS, TE SHRADER, G ROCAP, A VARSHAVSKY Science 29 Nov 1991: Vol. 254, Issue 5036, pp. 1374-1377 DOI: 10.1126/science.1962196
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:107997
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:10 Feb 2021 23:48
Last Modified:16 Nov 2021 19:08

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