A Caltech Library Service

An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme

Dohmen, R. Jürgen and Stappen, Reiner and McGrath, John P. and Forrová, Helena and Kolarov, Jordan and Goffeau, André and Varshavsky, Alexander (1995) An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme. Journal of Biological Chemistry, 270 (30). pp. 18099-18109. ISSN 0021-9258. doi:10.1074/jbc.270.30.18099.

[img] PDF - Published Version
Creative Commons Attribution Non-commercial No Derivatives.


Use this Persistent URL to link to this item:


Ubiquitin (Ub) activation by the Ub-activating (E1) enzyme is the initial and essential step common to all of the known processes that involve post-translational conjugation of Ub to itself or other proteins. The “activated” Ub, linked via a thioester bond to a specific cysteine residue of E1 enzyme, can be transferred to a cysteine residue in one of several Ub-conjugating (E2) enzymes, which catalyze the formation of isopeptide bonds between the C-terminal glycine of Ub and lysine residues of acceptor proteins. In the yeast Saccharomyces cerevisiae, a 114-kDa E1 enzyme is encoded by an essential gene termed UBA1 (McGrath, J. P., Jentsch, S., and Varshavsky, A.(1991) EMBO J. 10, 227-236). We describe the isolation and analysis of another essential gene, termed UBA2, that encodes a 71-kDa protein with extensive sequence similarities to both the UBA1-encoded yeast E1 and E1 enzymes of other organisms. The regions of similarities between Uba1p and Uba2p encompass a putative ATP-binding site as well as a sequence that is highly conserved between the known E1 enzymes and contains the active-site cysteine of E1. This cysteine is shown to be required for an essential function of Uba2p, suggesting that Uba2p-catalyzed reactions involve a transient thioester bond between Uba2p and either Ub or another protein. Uba2p is located largely in the nucleus. The putative nuclear localization signal of Uba2p is near its C terminus. The Uba1p (E1 enzyme) and Uba2p cannot complement each others essential functions even if their subcellular localization is altered by mutagenesis. Uba2p appears to interact with itself and several other S. cerevisiae proteins with apparent molecular masses of 52, 63, 87, and 120 kDa. Uba2p is multiubiquitinated in vivo, suggesting that at least a fraction of Uba2p is metabolically unstable. Uba2p is likely to be a component of the Ub system that functions as either an E2 or E1/E2 enzyme.

Item Type:Article
Related URLs:
URLURL TypeDescription
Varshavsky, Alexander0000-0002-4011-258X
Additional Information:© 1995 American Society for Biochemistry and Molecular Biology. Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0). Received for publication, March 30, 1995, and in revised form, May 18, 1995. This work was supported by a grant from the Bundesministerium für Bildung und Forschung (Förderkennzeichen 0316711) (to R. J. D.) and by Grant GM31530 from the National Institutes of Health (to A. V.). The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. The nucleotide sequence(s) reported in this paper has been submitted to the GenBank™/EMBL Data Bank with accession number(s) Z48725. We thank D. Finley, M. Ellison, K. Madura, V. Chau, M. Hochstrasser, E. Johnson, N. Johnsson, N. Schnell, P. Sherwood, and R. Young for strains, plasmids, DNA libraries, and other reagents. We are grateful to C. Moore and M. del Olmo for sharing their results with us before publication.
Funding AgencyGrant Number
Bundesministerium für Bildung und Forschung (BMBF)0316711
Issue or Number:30
Record Number:CaltechAUTHORS:20210211-152331529
Persistent URL:
Official Citation:R. Jürgen Dohmen, Reiner Stappen, John P. McGrath, Helena Forrová, Jordan Kolarov, André Goffeau, Alexander Varshavsky, An Essential Yeast Gene Encoding a Homolog of Ubiquitin-activating Enzyme, Journal of Biological Chemistry, Volume 270, Issue 30, 1995, Pages 18099-18109, ISSN 0021-9258,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108016
Deposited By: George Porter
Deposited On:12 Feb 2021 15:21
Last Modified:16 Nov 2021 19:08

Repository Staff Only: item control page