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Imaging Biopolymers In Vivo Directly with Cryo-ET: Trim5 Nets and IRE-1 Helices

Jensen, Grant (2021) Imaging Biopolymers In Vivo Directly with Cryo-ET: Trim5 Nets and IRE-1 Helices. Biophysical Journal, 120 (3, Supp. 1). 1a. ISSN 0006-3495.

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In the last ten years electron cryotomography (cryo-ET) has made it possible to visualize large macromolecular assemblies inside intact cells in a near-native, “frozen-hydrated” state in 3-D to a few nanometers resolution. increasingly, atomic models of individual proteins and smaller complexes obtained by X-ray crystallography, NMR spectroscopy, or other methods can be fit into cryotomograms to reveal how the various pieces work together inside cells. A few good pictures is therefore sometimes all that is really needed to distinguish between competing models. To illustrate these points, I will present examples of current results from our recent work on the HIV-restriction protein Trim5α and the unfolded protein response sensor IRE1α, both of which form polymers inside cells. Trim5α polymerizes into hexagonal lattices near sites of macroautophagy. IRE1α polymerizes into left-handed double helices inside thin ER tubules. Time permitting, I will also describe ongoing technology development efforts that promise to dramatically expand the applicability of cryo-ET.

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Jensen, Grant0000-0003-1556-4864
Additional Information:© 2021 Biophysical Society.
Issue or Number:3, Supp. 1
Record Number:CaltechAUTHORS:20210216-140505110
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Official Citation:Grant Jensen, Imaging Biopolymers In Vivo Directly with Cryo-ET: Trim5 Nets and IRE-1 Helices, Biophysical Journal, Volume 120, Issue 3, Supplement 1, 2021, Page 1a, ISSN 0006-3495,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108077
Deposited By: George Porter
Deposited On:16 Feb 2021 22:34
Last Modified:16 Feb 2021 22:34

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