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Imaging-based screens of pool-synthesized cell libraries

Lawson, Michael and Elf, Johan (2021) Imaging-based screens of pool-synthesized cell libraries. Nature Methods, 18 (4). pp. 358-365. ISSN 1548-7091. doi:10.1038/s41592-020-01053-8. https://resolver.caltech.edu/CaltechAUTHORS:20210223-111436775

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Abstract

Mapping a genetic perturbation to a change in phenotype is at the core of biological research. Advances in microscopy have transformed these studies, but they have largely been confined to examining a few strains or cell lines at a time. In parallel, there has been a revolution in creating synthetic libraries of genetically altered cells with relative ease. Here we describe methods that combine these powerful tools to perform live-cell imaging of pool-generated strain libraries for improved biological discovery.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41592-020-01053-8DOIArticle
https://rdcu.be/cfH20PublisherFree ReadCube access
ORCID:
AuthorORCID
Lawson, Michael0000-0002-2868-733X
Elf, Johan0000-0001-5522-1810
Additional Information:© 2021 Nature Publishing Group. Received 12 May 2020; Accepted 21 December 2020; Published 15 February 2021. We thank D. Jones, P. Blainey, B. Huber, M. Polonsky and L. Ombelets for critical and constructive reading of the manuscript. The work was funded by the Knut and Alice Wallenberg Foundation (KAW). Competing interests: J.E. has a patent on in situ genotyping after phenotyping (US patent 10,570,437). Peer review information: Rita Strack was the primary editor on this article and managed its editorial process and peer review in collaboration with the rest of the editorial team.
Funders:
Funding AgencyGrant Number
Knut and Alice Wallenberg FoundationUNSPECIFIED
Subject Keywords:Biological techniques; Biotechnology
Issue or Number:4
DOI:10.1038/s41592-020-01053-8
Record Number:CaltechAUTHORS:20210223-111436775
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210223-111436775
Official Citation:Lawson, M., Elf, J. Imaging-based screens of pool-synthesized cell libraries. Nat Methods 18, 358–365 (2021). https://doi.org/10.1038/s41592-020-01053-8
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108152
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Feb 2021 22:55
Last Modified:07 Apr 2021 19:15

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