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Microbial succession and dynamics in meromictic Mono Lake, California

Phillips, Alexandra A. and Speth, Daan R. and Miller, Laurence G. and Wang, Xingchen T. and Wu, Fenfang and Medeiros, Patricia M. and Monteverde, Danielle R. and Osburn, Magdalena R. and Berelson, William M. and Betts, Hannah L. and Wijker, Reto S. and Mullin, Sean W. and Johnson, Hope A. and Orphan, Victoria J. and Fischer, Woodward W. and Sessions, Alex L. (2021) Microbial succession and dynamics in meromictic Mono Lake, California. Geobiology . ISSN 1472-4677. (In Press) https://resolver.caltech.edu/CaltechAUTHORS:20210225-154113785

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Abstract

Mono Lake is a closed‐basin, hypersaline, alkaline lake located in Eastern Sierra Nevada, California, that is dominated by microbial life. This unique ecosystem offers a natural laboratory for probing microbial community responses to environmental change. In 2017, a heavy snowpack and subsequent runoff led Mono Lake to transition from annually mixed (monomictic) to indefinitely stratified (meromictic). We followed microbial succession during this limnological shift, establishing a two‐year (2017–2018) water‐column time series of geochemical and microbiological data. Following meromictic conditions, anoxia persisted below the chemocline and reduced compounds such as sulfide and ammonium increased in concentration from near 0 to ~400 and ~150 µM, respectively, throughout 2018. We observed significant microbial succession, with trends varying by water depth. In the epilimnion (above the chemocline), aerobic heterotrophs were displaced by phototrophic genera when a large bloom of cyanobacteria appeared in fall 2018. Bacteria in the hypolimnion (below the chemocline) had a delayed, but systematic, response reflecting colonization by sediment “seed bank” communities. Phototrophic sulfide‐oxidizing bacteria appeared first in summer 2017, followed by microbes associated with anaerobic fermentation in spring 2018, and eventually sulfate‐reducing taxa by fall 2018. This slow shift indicated that multi‐year meromixis was required to establish a sulfate‐reducing community in Mono Lake, although sulfide oxidizers thrive throughout mixing regimes. The abundant green alga Picocystis remained the dominant primary producer during the meromixis event, abundant throughout the water column including in the hypolimnion despite the absence of light and prevalence of sulfide. Our study adds to the growing literature describing microbial resistance and resilience during lake mixing events related to climatic events and environmental change.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1111/gbi.12437DOIArticle
ORCID:
AuthorORCID
Phillips, Alexandra A.0000-0001-5959-5238
Speth, Daan R.0000-0002-2361-5935
Miller, Laurence G.0000-0002-7807-3475
Wang, Xingchen T.0000-0001-5316-789X
Wu, Fenfang0000-0003-1134-280X
Medeiros, Patricia M.0000-0001-6818-2603
Monteverde, Danielle R.0000-0002-0198-8220
Osburn, Magdalena R.0000-0001-9180-559X
Berelson, William M.0000-0002-1526-3802
Wijker, Reto S.0000-0001-5104-9849
Mullin, Sean W.0000-0002-6225-3279
Johnson, Hope A.0000-0001-6721-3340
Orphan, Victoria J.0000-0002-5374-6178
Fischer, Woodward W.0000-0002-8836-3054
Sessions, Alex L.0000-0001-6120-2763
Additional Information:© 2021 The Authors. Geobiology published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. Version of Record online: 25 February 2021; Manuscript accepted: 08 February 2021; Manuscript revised: 18 November 2020; Manuscript received: 29 May 2020. This research was supported by the International Geobiology Course in 2017, 2018, and 2019. Funding for the course was provided by the Agouron Institute (Award #12520044) and the Simons Foundation (Award #12520045). We would like to thank Blake Stamps for the use of his code that was modified to generate our 16S OTU heatmaps. We also acknowledge Ronald Oremland for shipping an aliquot of Picocystis culture. We thank Sujung Lim and Grayson Chadwick for early conversations on 16S rRNA data, Usha Lingappa for her expertise on Cyanobacteria, and Aaron Martinez for his helpful dialogue on fatty acids. We are grateful to Jared Leadbetter for continuous conversations about the microbial community. We acknowledge Ashley Shade for her helpful dialogue on microbial succession in lakes. We thank Miquela Ingalls for comments and suggestions on the manuscript and her help with sample processing in the field. Additionally, we thank other field assistants: Jodi Blum, Elise Wilkes, Yonaton Goldsmith, and Barbara Ratschbacher. We would like to thank Tim Hollibaugh for use of his Seabird CTD. We thank Melissa Miller from the Oceanographic Data Facility at Scripps Institution of Oceanography for measuring nutrient concentrations and Joshua West at the University of Southern California for measuring trace metals. We acknowledge members of the Agouron International Geobiology Course from 2017 and 2018, especially students who worked directly with Mono Lake water‐column samples: Katherine Fullerton, Rui Bao, Dawson Fairbanks, Michael Wells, Sergio Parra, Qingzeng Zhu, Bridget Conley, and Maria Figueroa. We thank the administrative team for the geobiology courses in 2017 and 2018, including Julie Lee and Ann Close. We also acknowledge course TAs, including Lizzy Trower, John Magyar, Eryn Eitel, and Elise Wilkes. We would like to thank the Agouron Institute and the Simons Foundation for funding the International Geobiology Course and this research. We would also like to acknowledge the Mono Lake Committee and the LA Department of Water and Power for detailed, public records on lake levels and other Mono Lake parameters. Data Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request. 16S rRNA amplicon sequencing data is available in GenBank under BioProject accession number PRJNA702881.
Funders:
Funding AgencyGrant Number
Agouron Institute12520044
Simons Foundation12520045
Subject Keywords:environmental microbiology; geochemistry; limnology; microbial ecology; microbial succession
Record Number:CaltechAUTHORS:20210225-154113785
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210225-154113785
Official Citation:Phillips, AA, Speth, DR, Miller, LG, et al; Geobiology Course 2017; Geobiology Course 2018. Microbial succession and dynamics in meromictic Mono Lake, California. Geobiology. 2021; 00: 1–18. https://doi.org/10.1111/gbi.12437
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108226
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:25 Feb 2021 23:52
Last Modified:25 Feb 2021 23:52

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