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Suppressing mosquito populations with precision guided sterile males

Li, Ming and Yang, Ting and Bui, Michelle and Gamez, Stephanie and Wise, Tyler and Kandul, Nikolay P. and Liu, Junru and Alcantara, Lenissa and Lee, Haena and Edula, Jyotheeswara R. and Raban, Robyn and Zhan, Yinpeng and Wang, Yijin and DeBeaubien, Nick and Chen, Jieyan and Sanchez C., Hector M. and Bennett, Jared B. and Antoshechkin, Igor and Montell, Craig and Marshall, John M. and Akbari, Omar S. (2021) Suppressing mosquito populations with precision guided sterile males. Nature Communications, 12 . Art. No. 5374. ISSN 2041-1723. doi:10.1038/s41467-021-25421-w. https://resolver.caltech.edu/CaltechAUTHORS:20210308-122724699

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Abstract

The mosquito Aedes aegypti is the principal vector for arboviruses including dengue/yellow fever, chikungunya, and Zika virus, infecting hundreds of millions of people annually. Unfortunately, traditional control methodologies are insufficient, so innovative control methods are needed. To complement existing measures, here we develop a molecular genetic control system termed precision-guided sterile insect technique (pgSIT) in Aedes aegypti. PgSIT uses a simple CRISPR-based approach to generate flightless females and sterile males that are deployable at any life stage. Supported by mathematical models, we empirically demonstrate that released pgSIT males can compete, suppress, and even eliminate mosquito populations. This platform technology could be used in the field, and adapted to many vectors, for controlling wild populations to curtail disease in a safe, confinable, and reversible manner.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1038/s41467-021-25421-wDOIArticle
https://doi.org/10.1101/2021.03.05.434167DOIDiscussion Paper
https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA699282Related ItemData
ORCID:
AuthorORCID
Li, Ming0000-0002-7578-4968
Yang, Ting0000-0001-7201-4231
Gamez, Stephanie0000-0003-0665-0066
Liu, Junru0000-0002-6198-8150
Raban, Robyn0000-0002-5648-6770
Antoshechkin, Igor0000-0002-9934-3040
Montell, Craig0000-0001-5637-1482
Marshall, John M.0000-0003-0603-7341
Akbari, Omar S.0000-0002-6853-9884
Alternate Title:Eliminating Mosquitoes with Precision Guided Sterile Males
Additional Information:© The Author(s) 2021. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. Received 26 March 2021; Accepted 23 July 2021; Published 10 September 2021. We thank Judy Ishikawa for helping with mosquito husbandry. This work was supported by funding from a DARPA Safe Genes Program Grant (HR0011-17-2-0047) and an NIH award (R01AI151004) awarded to O.S.A. support to CM from the “U.S. Army Research Office and accomplished under cooperative agreement W911NF-19-2-0026 for the Institute for Collaborative Biotechnologies”, support to J.M.M. from the Innovative Genomics Institute, and an NIH award (R56-AI153334). The views, opinions, and/or findings expressed are those of the authors and should not be interpreted as representing the official views or policies of the U.S. government. Data availability: Complete sequence maps assembled in the study are deposited at Addgene.org (#164846 - #164848) and available for distribution (Supplementary Table 18). All Illumina and Nanopore sequencing data have been deposited to the NCBI-SRA https://www.ncbi.nlm.nih.gov/sra/?term=PRJNA699282. All data used to generate figures are provided in the Supplementary Materials/Source Data files (Supplementary Tables 1–18). Generated Aedes transgenic lines are available upon request to O.S.A. Source data are provided with this paper. These authors contributed equally: Ming Li, Ting Yang. Author Contributions: O.S.A. and M.L. conceptualized and designed experiments; T.Y., J.L., L.A., and S.G. performed molecular analyses; M.L., J.R.E., T.W., H.L., and M.B. performed genetic experiments; Y.Z, Y.W., N.D., J.C., and C.M. performed behavioral experiments; J.B., H.M.S.C., and J.M.M. performed mathematical modeling; I.A. performed bioinformatics: O.S.A., M.L., N.P.K., and R.R. analyzed and compiled the data. All authors contributed to writing and approved the final manuscript. Competing interests: O.S.A. is a founder of Agragene, Inc. and has an equity interest. The terms of this arrangement have been reviewed and approved by the University of California, San Diego in accordance with its conflict of interest policies. All remaining authors declare no competing interests. Peer review information: This article has been peer reviewed as part of Springer Nature’s Guided Open Access initiative. Nature Communications thanks Marcelo Jacobs-Lorena, Michael Smanski, Jeff Sekelsky and the other, anonymous, reviewer(s) for their contribution to the peer review of this work.
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)HR0011-17-2-0047
NIHR01AI151004
Army Research Office (ARO)W911NF-19-2-0026
NIHR56-AI153334
Subject Keywords:Assay systems; CRISPR-Cas9 genome editing
DOI:10.1038/s41467-021-25421-w
Record Number:CaltechAUTHORS:20210308-122724699
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210308-122724699
Official Citation:Li, M., Yang, T., Bui, M. et al. Suppressing mosquito populations with precision guided sterile males. Nat Commun 12, 5374 (2021). https://doi.org/10.1038/s41467-021-25421-w
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108343
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Mar 2021 20:45
Last Modified:23 Sep 2021 22:18

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