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Point Mutations Within and Outside the Homeodomain Identify Sequences Required for proboscipedia Homeotic Function in Drosophila

Benassayag, Corinne and Boube, Muriel and Seroude, Laurent and Cribbs, David L. (1997) Point Mutations Within and Outside the Homeodomain Identify Sequences Required for proboscipedia Homeotic Function in Drosophila. Genetics, 146 (3). pp. 939-949. ISSN 0016-6731. PMCID PMC1208062. https://resolver.caltech.edu/CaltechAUTHORS:20210310-082146482

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Abstract

The Drosophila homeotic gene proboscipedia (pb) encodes a homeodomain protein homologous to vertebrate HoxA2/B2 required for adult mouthparts formation. A transgenic Hsp70-pb (HSPB) element that rescues pb mutations also induces the dominant transformation of antennae to maxillary palps. To identify sequences essential to PB protein function, we screened for EMS-induced HSPB mutations leading to phenotypic reversion of the HSPB transformation. Ten revertants harbor identified point mutations in HSPB coding sequences. The point mutations that remove all detectable phenotypes in vivo reside either within the homeodomain or, more unexpectedly,in evolutionarily nonconserved regions outside the homeodomain. Two independent homeodomain mutations that change the highly conserved Arginine-5 in the N-terminal hinge show effects on adult eye development, suggesting a previously unsuspected role for Arg5 in functional specificity. Three additional revertant mutations outside the homeodomain reduce but do not abolish PB⁺ activity, identifylng protein elements that contribute quantitatively to pb function. This in vivo analysis shows that apart from the conserved motifs of PB, other elements throughout the protein make important contributions to homeotic function.


Item Type:Article
Related URLs:
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https://www.genetics.org/content/146/3/939.longPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1208062PubMed CentralArticle
Additional Information:© 1997 by the Genetics Society of America. Received January 29, 1997. Accepted March 21, 1997. We thank STEPHANE NOSELLI, BRUNO GLISE, ALAIN VINCENT and HENRI-MARC BOURBON for their critical readings of the manuscript. C.B., M.B. and L.S. were supported by graduate fellowships from the Ministere de l'Enseignement Superieur et de la Recherche (MESR); M.B. and L.S. also benefited from graduate training fellowships from the Association pour la Recherche contre le Cancer (ARC). This work was made possible by ongoing funding from the Centre National de Recherche Scientifique and grants from the ARC and the MESR.
Funders:
Funding AgencyGrant Number
Ministère de l'Enseignement supérieur, de la Recherche et de l'Innovation (MESRI)UNSPECIFIED
Association pour la Recherche contre le CancerUNSPECIFIED
Centre National de la Recherche Scientifique (CNRS)UNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC1208062
Record Number:CaltechAUTHORS:20210310-082146482
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210310-082146482
Official Citation:Point Mutations Within and Outside the Homeodomain Identify Sequences Required for proboscipedia Homeotic Function in Drosophila. Corinne Benassayag, Muriel Boube, Laurent Seroude and David L. Cribbs. GENETICS July 1, 1997 vol. 146 no. 3 939-949
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108377
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:10 Mar 2021 20:17
Last Modified:10 Mar 2021 20:17

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