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Genetic analysis of transvection effects involving cis-regulatory elements of the Drosophila Ultrabithorax gene

Micol, José Luis and Castelli-Gair, James E. and García-Bellido, Antonio (1990) Genetic analysis of transvection effects involving cis-regulatory elements of the Drosophila Ultrabithorax gene. Genetics, 126 (2). pp. 365-373. ISSN 0016-6731. PMCID PMC1204191. https://resolver.caltech.edu/CaltechAUTHORS:20210310-093914044

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Abstract

The Ultrabithorax (Ubx) gene of Drosophila melanogaster contains two functionally distinguishable regions: the protein-coding Ubx transcription unit and, upstream of it, the transcribed but nonprotein-coding bxd region. Numerous recessive, partial loss-of-function mutations which appear to be regulatory mutations map within the bxd region and within the introns of the Ubx transcription unit. In addition, mutations within the Ubx unit exons are known and most of these behave as null alleles. Ubx1 is one such allele. We have confirmed that, although the Ubx1 allele does not produce detectable Ubx proteins (UBX), it does retain other genetic functions detectable by their effects on the expression of a paired, homologous Ubx allele, i.e., by transvection. We have extended previous analyses made by E. B. Lewis by mapping the critical elements of the Ubx gene which participate in transvection effects. Our results show that the Ubx1 allele retains wild-type functions whose effectiveness can be reduced (1) by additional cis mutations in the bxd region or in introns of the Ubx transcription unit, as well as (2) by rearrangements disturbing pairing between homologous Ubx genes. Our results suggest that those remnant functions in Ubx1 are able to modulate the activity of the allele located in the homologous chromosome. We discuss the normal cis regulatory role of these functions involved in trans interactions between homologous Ubx genes, as well as the implications of our results for the current models on transvection.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://www.genetics.org/content/126/2/365.longPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1204191PubMed CentralArticle
ORCID:
AuthorORCID
Micol, José Luis0000-0002-0396-1750
Additional Information:© 1990 by the Genetics Society of America. Received January 20, 1990; Accepted for publication June 8, 1990. Thanks are due to E. B. LEWIS, G. MORATA and W. BENDER for providing stocks, to M. P. CAPDEVILA for discussions, and to F. J. CALZONE, E. H. DAVIDSON, E. B. LEWIS, D. R. MATHOG, E. M. MEYEROWITZ and J. E. MINOR, JR., for comments on the manuscript. This work was supported by Comisión Asesora de lnvestigación Científica y Técnica, Comisión Interministerial de Ciencia y Tecnología, Fundación Ramon Areces and Fundación Juan March. J.L.M. was postdoctoral fellow of Fundación Juan March. J.E.C.-G. was fellow of British Council-Consejo Superior de Investigaciones Científicas.
Funders:
Funding AgencyGrant Number
Comisión Asesora de lnvestigación Científica y TécnicaUNSPECIFIED
Comisión Interministerial de Ciencia y Tecnología (CICYT)UNSPECIFIED
Fundación Ramon ArecesUNSPECIFIED
Fundación Juan MarchUNSPECIFIED
Consejo Superior de Investigaciones Científicas (CSIC)UNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC1204191
Record Number:CaltechAUTHORS:20210310-093914044
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210310-093914044
Official Citation:Genetic analysis of transvection effects involving cis-regulatory elements of the Drosophila Ultrabithorax gene. J L Micol, J E Castelli-Gair and A García-Bellido. GENETICS October 1, 1990 vol. 126 no. 2 365-373
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108381
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Mar 2021 16:57
Last Modified:11 Mar 2021 16:57

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