CaltechAUTHORS
  A Caltech Library Service

Spatial Control of Probiotic Bacteria in the Gastrointestinal Tract Assisted by Magnetic Particles

Buss, Marjorie T. and Ramesh, Pradeep and English, Max Atticus and Lee-Gosselin, Audrey and Shapiro, Mikhail G. (2021) Spatial Control of Probiotic Bacteria in the Gastrointestinal Tract Assisted by Magnetic Particles. Advanced Materials, 33 (17). Art. No. 2007473. ISSN 0935-9648. doi:10.1002/adma.202007473. https://resolver.caltech.edu/CaltechAUTHORS:20210315-102208370

[img] PDF - Supplemental Material
See Usage Policy.

3MB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20210315-102208370

Abstract

Engineered probiotics have the potential to diagnose and treat a variety of gastrointestinal (GI) diseases. However, these exogenous bacterial agents have limited ability to effectively colonize specific regions of the GI tract due to a lack of external control over their localization and persistence. Magnetic fields are well suited to providing such control, since they freely penetrate biological tissues. However, they are difficult to apply with sufficient strength to directly manipulate magnetically labeled cells in deep tissue such as the GI tract. Here, it is demonstrated that a composite biomagnetic material consisting of microscale magnetic particles and probiotic bacteria, when orally administered and combined with an externally applied magnetic field, enables the trapping and retention of probiotic bacteria within the GI tract of mice. This technology improves the ability of these probiotic agents to accumulate at specific locations and stably colonize without antibiotic treatment. By enhancing the ability of GI‐targeted probiotics to be at the right place at the right time, cellular localization assisted by magnetic particles (CLAMP) adds external physical control to an important emerging class of microbial theranostics.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1002/adma.202007473DOIArticle
https://resolver.caltech.edu/CaltechAUTHORS:20210505-072449111Related ItemCover Story
ORCID:
AuthorORCID
Lee-Gosselin, Audrey0000-0002-2431-2741
Shapiro, Mikhail G.0000-0002-0291-4215
Additional Information:© 2021 Wiley-VCH GmbH. Issue Online: 27 April 2021; Version of Record online: 11 March 2021; Manuscript revised: 19 January 2021; Manuscript received: 01 November 2020. M.T.B. and P.R. contributed equally to this work. This work was supported by the Pew Charitable Trust, the Packard Foundation, and the Heritage Medical Research Institute. M.T.B. was supported by an NSF GRFP fellowship. P.R. was supported by a Rosen center for Bioengineering predoctoral fellowship. The authors would like to thank Dr. Said Bogatyrev and Dr. Asher Preska Steinberg for their helpful advice, the Caltech Flow Cytometry Facility for assistance with flow cytometry, and Ivetta Vorobyova for her help with the X‐ray CT studies. The authors declare no conflict of interest. Data Availability Statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.
Group:Heritage Medical Research Institute, Rosen Bioengineering Center
Funders:
Funding AgencyGrant Number
Pew Charitable Trust91127025
David and Lucile Packard FoundationUNSPECIFIED
Heritage Medical Research InstituteUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
Donna and Benjamin M. Rosen Bioengineering CenterUNSPECIFIED
Subject Keywords:biomaterials, magnetic control, magnetic particles, microbiome, probiotics
Issue or Number:17
DOI:10.1002/adma.202007473
Record Number:CaltechAUTHORS:20210315-102208370
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210315-102208370
Official Citation:Buss, M. T., Ramesh, P., English, M. A., Lee‐Gosselin, A., Shapiro, M. G., Spatial Control of Probiotic Bacteria in the Gastrointestinal Tract Assisted by Magnetic Particles. Adv. Mater. 2021, 33, 2007473. https://doi.org/10.1002/adma.202007473
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108426
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Mar 2021 02:06
Last Modified:05 May 2021 14:28

Repository Staff Only: item control page