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Long molecules of free DNA in the sheared chromatin preparation

Varshavsky, A. J. and Ilyin, Yu. V. and Georgiev, G. P. (1973) Long molecules of free DNA in the sheared chromatin preparation. Molecular Biology Reports, 1 (4). pp. 201-207. ISSN 0301-4851. doi:10.1007/bf00357642.

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Hydrodynamic shearing of chromatin in the presence of Mg²⁺ ions produces two discrete types of particles: (1) molecules of completely free DNA which comprise 20–23% of the total DNA and (2) histone-covered DNA molecules which contain all five histone fractions. The average length of free DNA molecules depends on the intensity of shearing and can be as high as 1000 base pairs or more. Shearing of chromatin in the absence of Mg²⁺ produces a heterogeneous population of DNP particles; no free DNA is liberated. However, the addition of Mg²⁺ to this preparation results in appearance of free DNA molecules and in a complete restoration of the above ‘bimodal’ distribution. These findings support a previously proposed ‘asymmetric hairpin’ model of DNA packing in the chromatin [1–3].

Item Type:Article
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Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1973 Springer. Received 05 October 1973.
Subject Keywords:Base Pair; Average Length; Heterogeneous Population; Complete Restoration; Discrete Type
Issue or Number:4
Record Number:CaltechAUTHORS:20210317-085119478
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Official Citation:Varshavsky, A.J., Ilyin, Y.V. & Georgiev, G.P. Long molecules of free DNA in the sheared chromatin preparation. Molecular Biology Reports 1, 201–207 (1973).
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108461
Deposited By: Tony Diaz
Deposited On:19 Mar 2021 00:51
Last Modified:19 Mar 2021 00:51

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