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Studies on chromatin. IV. Evidence for a toroidal shape of chromatin subunits

Varshavsky, A. J. and Bakayev, V. V. (1975) Studies on chromatin. IV. Evidence for a toroidal shape of chromatin subunits. Molecular Biology Reports, 2 (3). pp. 247-254. ISSN 0301-4851. doi:10.1007/bf00356995. https://resolver.caltech.edu/CaltechAUTHORS:20210317-090059214

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Abstract

Electron microscopy of purified chromatin subunits (ν-bodies [17] or nucleosomes [2] revealed a hole or at least a deep indentation in the globular nucleosome. A hole in the nucleosome was visualized using rotatory shadowing with platinum-palladium or more directly, by negative staining with sodium phosphotungstate. The diameter of the hole as measured from negatively stained samples is 10–25Å. The external diameter of the negatively stained nucleosome equals 75±15Å. Although most of the data are formally compatible with either a hole or a deep indentation in the nucleosome, some views of the particles in the negatively stained samples suggest a hole rather than an indentation. The possible significance of a toroidal structure of the chromatin subunit is discussed in the accompanying paper [3].


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1007/bf00356995DOIArticle
ORCID:
AuthorORCID
Varshavsky, A. J.0000-0002-4011-258X
Additional Information:© 1975 Springer. Received 15 August 1975.
Subject Keywords:Sodium; Electron Microscopy; External Diameter; Negative Staining; Deep Indentation
Issue or Number:3
DOI:10.1007/bf00356995
Record Number:CaltechAUTHORS:20210317-090059214
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210317-090059214
Official Citation:Varshavsky, A.J., Bakayev, V.V. Studies on chromatin. IV. Evidence for a toroidal shape of chromatin subunits. Molecular Biology Reports 2, 247–254 (1975). https://doi.org/10.1007/BF00356995
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108464
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Mar 2021 00:47
Last Modified:19 Mar 2021 00:47

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