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Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster

Dushay, Mitchell S. and Konopka, Ronald J. and Orr, Dominic and Greenacre, Mary L. and Kyriacou, Charalambos P. and Rosbash, Michael and Hall, Jeffrey C. (1990) Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster. Genetics, 125 (3). pp. 557-578. ISSN 0016-6731. PMCID PMC1204083. https://resolver.caltech.edu/CaltechAUTHORS:20210318-144523335

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Abstract

Clock is a semidominant X-linked mutation that results in shortening the period of Drosophila melanogaster's free-running locomotor activity rhythm from ca. 24.0 to ca. 22.5 hr. This mutation similarly shortened the phase response curve, determined by resetting activity rhythms with light pulses. Eclosion peaks for Clk cultures were separated by only 22.5 hr instead of the normal 24 hr. Clk was mapped close to, but separable from, another rhythm mutation--period01--by recombination. The estimated distance between these two mutations was short enough to suggest that Clk could be a per allele. If this is the case, the new mutant is unique in that it, unlike other per variants, is associated with essentially normal 1-min courtship song rhythms when Clk is expressed in males. Also, the new rhythm variant could not, in contrast to a short-period per mutation, have its effects on free-running activity rhythms uncovered by deletions. This result, and the lack of coverage of Clk's effects by duplications, suggest that it is not a simple hypomorphic or amorphic mutation.


Item Type:Article
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https://www.genetics.org/content/125/3/557.longPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1204083PubMed CentralArticle
Additional Information:© 1990 Genetics Society of America. Manuscript received December 19, 1989; Accepted for publication March 30, 1990. We thank MELANIE HAMBLEN-COYLE for her extensive participation in the activity rhythm monitoring experiments, and ADAM HURWITZ and JOHN R. NAMBU for assisting with Clk mapping. We appreciate the help with rhythm analyses provided by DAVID A. WHEELER, HAROLD B. DOWSE, and MARC J. VAN DEN BERG (the latter two of whom graciously donated software). We are grateful for discussions with JEAN-MAURICE DURA (who also produced and gave us a marked control stock) and for comments on the manuscript from VALERIE K. L. MERRILL and THOMAS PREAT. This research was supported by National Institutes of Health grants: GM-33205 to M.R. and J.C.H., and GM-35783 to RJ.K.
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Funding AgencyGrant Number
NIHGM-33205
NIHGM-35783
Issue or Number:3
PubMed Central ID:PMC1204083
Record Number:CaltechAUTHORS:20210318-144523335
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210318-144523335
Official Citation:Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster. M S Dushay, R J Konopka, D Orr, M L Greenacre, C P Kyriacou, M Rosbash and J C Hall. GENETICS July 1, 1990 vol. 125 no. 3 557-578
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108490
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:19 Mar 2021 00:58
Last Modified:19 Mar 2021 00:58

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