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Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes

Kaplan, Mohammed and Wang, Yuhang and Chreifi, Georges and Zhang, Lujia and Chang, Yi-Wei and Jensen, Grant J. (2021) Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes. Journal of Molecular Biology, 433 (13). Art. No. 167004. ISSN 0022-2836. doi:10.1016/j.jmb.2021.167004. https://resolver.caltech.edu/CaltechAUTHORS:20210423-164854049

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Abstract

The bacterial flagellum consists of a long extracellular filament that is rotated by a motor embedded in the cell envelope. While flagellar assembly has been extensively studied, the disassembly process remains less well understood. In addition to the programmed flagellar ejection that occurs during the life cycle of Caulobacter crescentus, we and others have recently shown that many bacterial species lose their flagella under starvation conditions, leaving relic structures in the outer membrane. However, it remains unknown whether the programmed flagellar ejection of C. crescentus leaves similar relics or not. Here, we imaged the various stages of the C. crescentus life cycle using electron cryo-tomography (cryo-ET) and found that flagellar relic subcomplexes, akin to those produced in the starvation-induced process, remain as a result of flagellar ejection during cell development. This similarity suggests that the programmed flagellar ejection of C. crescentus might share a common evolutionary path with the more general, and likely more ancient (Kaplan et al., 2020), starvation-related flagellar loss.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.jmb.2021.167004DOIArticle
ORCID:
AuthorORCID
Kaplan, Mohammed0000-0002-0759-0459
Chreifi, Georges0000-0003-4194-1694
Zhang, Lujia0000-0003-3566-917X
Chang, Yi-Wei0000-0003-2391-473X
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2021 Elsevier. Received 11 December 2020, Revised 12 April 2021, Accepted 13 April 2021, Available online 20 April 2021. This project was funded by the NIH (grant RO1 AI127401 to G.J.J.) and a Baxter postdoctoral fellowship from Caltech to M.K. Cryo-ET work was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at the California Institute of Technology. We are grateful to Catherine Oikonomou for critically reading the manuscript. We thank Prof. Ariane Briegel for collecting some of the data and Songye Chen for technical support. We are grateful to the lab of Prof. Patrick Viollier (University of Geneva) for sending wild type and delta CapF strains. The C. crescentus DpleD strain (UJ4450) was kindly sent to us from the lab of Prof. Urs Jenal (University of Basel), and we also thank Prof. Urs Jenal for critically reading our manuscript. CRediT authorship contribution statement: Mohammed Kaplan: Conceptualization, Data curation, Data analysis, writing original draft and editing, Funding acquisition. Yuhang Wang: Data curation, Writing - review & editing. Georges Chreifi: Data curation, Writing - review & editing. Lujia Zhang: Data curation, Writing - review & editing. Yi-Wei Chang: Data curation, Writing - review & editing. Grant J. Jensen: Conceptualization, Data analysis, Writing - review & editing, Funding acquisition. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Funders:
Funding AgencyGrant Number
NIHRO1 AI127401
CaltechUNSPECIFIED
Subject Keywords:bacterial flagellar motor; flagellar ejection; disassembly; PL-subcomplexes; electron cryo-tomography
Issue or Number:13
DOI:10.1016/j.jmb.2021.167004
Record Number:CaltechAUTHORS:20210423-164854049
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210423-164854049
Official Citation:Mohammed Kaplan, Yuhang Wang, Georges Chreifi, Lujia Zhang, Yi-Wei Chang, Grant J. Jensen, Programmed Flagellar Ejection in Caulobacter crescentus Leaves PL-subcomplexes, Journal of Molecular Biology, Volume 433, Issue 13, 2021, 167004, ISSN 0022-2836, https://doi.org/10.1016/j.jmb.2021.167004. (https://www.sciencedirect.com/science/article/pii/S0022283621002059)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:108827
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:28 Apr 2021 15:12
Last Modified:12 May 2021 16:48

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