CaltechAUTHORS
  A Caltech Library Service

Transcriptional Response to a Dauer-Inducing Ascaroside Cocktail in Late L1 in C. elegans

Cohen, Sarah M. and Sun, Jessica J. and Schroeder, Frank C. and Sternberg, Paul W. (2021) Transcriptional Response to a Dauer-Inducing Ascaroside Cocktail in Late L1 in C. elegans. microPublication Biology, 2021 . Art. No. 000397. ISSN 2578-9430. PMCID PMC8116936. doi:10.17912/micropub.biology.000397. https://resolver.caltech.edu/CaltechAUTHORS:20210517-095926181

[img] PDF - Published Version
Creative Commons Attribution.

2MB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20210517-095926181

Abstract

C. elegans larvae choose between dauer diapause and immediate reproductive development in two phases, the first being a choice between L2 and L2d, and the second between dauer and L3 (Golden and Riddle 1984). We used RNA-seq to study the transcriptomic response of well-fed C. elegans when exposed to a cocktail of diapause-inducing ascarosides (ascr#2, ascr#3, and ascr#8) during the L1 larval stage when they first receive cues from their environment and then adapt via developmental programming (Kaplan et al. 2011). This research was performed in N2 wild-type worms and daf-22(ok693) mutants that lack an enzyme necessary for the biosynthesis of ascaroside pheromones (reviewed by Ludewig and Schroeder 2013). We found that early exposure to ascarosides triggers a pervasive transcriptional response that may drive diapause induction within as little as three hours. In N2, 362 genes were significantly differentially expressed at p = 0.001, while in daf-22, 1,007 genes were significantly differentially expressed at p = 0.001. The daf-22 mutant may have more significantly expressed genes when exposed to ascarosides indicating a stronger response as it has never encountered ascaroside signaling previously, even at low levels.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.17912/micropub.biology.000397DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116936PubMed CentralArticle
ORCID:
AuthorORCID
Cohen, Sarah M.0000-0002-5233-2280
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2021 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Received: April 29, 2021; Revision received: May 6, 2021; Accepted: May 6, 2021; Published: May 12, 2021. This work was supported by the Amgen Scholars Program at Caltech, by UF1NS111697 to PWS and by GM0088290 to FCS. Author Contributions: Sarah M Cohen: Conceptualization, Methodology, Formal analysis, Writing - original draft, Writing - review and editing, Data curation. Jessica J Sun: Visualization, Data curation, Formal analysis, Writing - review and editing. Frank C Schroeder: Funding acquisition, Project administration, Resources, Supervision, Writing - review and editing, Conceptualization. Paul W Sternberg: Funding acquisition, Project administration, Resources, Supervision, Writing - review and editing, Conceptualization. Reviewed By: Anonymous.
Funders:
Funding AgencyGrant Number
AmGen Scholars ProgramUNSPECIFIED
NIHUF1NS111697
NIHGM0088290
PubMed Central ID:PMC8116936
DOI:10.17912/micropub.biology.000397
Record Number:CaltechAUTHORS:20210517-095926181
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210517-095926181
Official Citation:Cohen, SM; Sun, JJ; Schroeder, FC; Sternberg, PW (2021). Transcriptional Response to a Dauer-Inducing Ascaroside Cocktail in Late L1 in C. elegans. microPublication Biology. 10.17912/micropub.biology.000397
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:109146
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 May 2021 17:56
Last Modified:17 May 2021 17:56

Repository Staff Only: item control page