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Quantitative examination of five stochastic cell-cycle and cell-size control models for Escherichia coli and Bacillus subtilis

Le Treut, Guillaume and Si, Fangwei and Li, Dongyang and Jun, Suckjoon (2021) Quantitative examination of five stochastic cell-cycle and cell-size control models for Escherichia coli and Bacillus subtilis. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20210607-115053460

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Abstract

We examine five quantitative models of the cell-cycle and cell-size control in Escherichia coli and Bacillus subtilis that have been proposed over the last decade to explain single-cell experimental data generated with high-throughput methods. After presenting the statistical properties of these models, we test their predictions against experimental data. Based on simple calculations of the defining correlations in each model, we first dismiss the stochastic Helmstetter-Cooper model and the Initiation Adder model, and show that both the Replication Double Adder and the Independent Double Adder model are more consistent with the data than the other models. We then apply a recently proposed statistical analysis method and obtain that the Independent Double Adder model is the most likely model of the cell cycle. By showing that the Replication Double Adder model is fundamentally inconsistent with size convergence by the adder principle, we conclude that the Independent Double Adder model is most consistent with the data and the biology of bacterial cell-cycle and cell-size control. Mechanistically, the Independent Adder Model is equivalent to two biological principles: (i) balanced biosynthesis of the cell-cycle proteins, and (ii) their accumulation to a respective threshold number to trigger initiation and division.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2021.06.06.447266DOIDiscussion Paper
ORCID:
AuthorORCID
Le Treut, Guillaume0000-0002-9842-2804
Jun, Suckjoon0000-0002-0139-4297
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. The authors have declared no competing interest.
Funders:
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20210607-115053460
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210607-115053460
Official Citation:Quantitative examination of five stochastic cell-cycle and cell-size control models for Escherichia coli and Bacillus subtilis Guillaume Le Treut, Fangwei Si, Dongyang Li, Suckjoon Jun bioRxiv 2021.06.06.447266; doi: https://doi.org/10.1101/2021.06.06.447266
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:109414
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:07 Jun 2021 22:00
Last Modified:07 Jun 2021 22:00

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