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Timing of meristem initiation and maintenance determines the morphology of fern gametophytes

Wu, Xiao and Yan, An and McAdam, Scott A. M. and Banks, Jo Ann and Zhang, Shaoling and Zhou, Yun (2021) Timing of meristem initiation and maintenance determines the morphology of fern gametophytes. Journal of Experimental Botany . ISSN 0022-0957. doi:10.1093/jxb/erab307. (In Press) https://resolver.caltech.edu/CaltechAUTHORS:20210621-170112415

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Abstract

The alternation of generations in land plants occurs between the sporophyte phase and the gametophyte phase. The sporophytes of seed plants develop self-maintained, multicellular meristems, and these meristems determine plant architecture. The gametophytes of seed plants lack meristems and are heterotrophic. In contrast, the gametophytes of seed-free vascular plants, including ferns, are autotrophic and free-living, developing meristems to sustain their independent growth and proliferation. Compared to meristems in the sporophytes of seed plants, the cellular mechanisms underlying meristem development in fern gametophytes remain largely unknown. Here, using confocal time-lapse live imaging and computational segmentation and quantification, we determined different patterns of cell divisions associated with the initiation and proliferation of two distinct types of meristems in fern gametophytes. Our results reveal how the simple timing of a switch between two meristems has considerable consequences for the divergent gametophyte morphologies of two closely related ferns from Pteridaceae (Pteris and Ceratopteris). Our result provides evolutionary insight into the function and regulation of gametophyte meristems in seed-free vascular plants.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1093/jxb/erab307DOIArticle
https://doi.org/10.1101/2021.06.14.448200DOIDiscussion Paper
ORCID:
AuthorORCID
McAdam, Scott A. M.0000-0002-9625-6750
Zhou, Yun0000-0003-4105-5174
Alternate Title:Meristem development in fern gametophytes
Additional Information:© The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model). Published: 28 June 2021. The authors thank the Purdue Bindley Bioscience Facility for the access of the ZEISS LSM880 confocal microscope. The authors also thank Prof. Elliot Meyerowitz at Caltech for the support and encouragement. This work was supported by Purdue University start-up and funds from Purdue Center for Plant Biology (to YZ) and by the NSF-IOS 1931114 (to JB and YZ). Author contributions: YZ conceived the research direction, XW performed the experiments, XW, AY, JB SM, SZ, and YZ analyzed and discussed the experimental results, AY performed computational analysis and quantification, YZ supervised the experiments, XW, JB and YZ wrote the manuscript, AY, SM and SZ revised the manuscript, and all authors approved the manuscript. Authors declare no conflict of interest. All the authors declare no conflict of interest.
Funders:
Funding AgencyGrant Number
Purdue UniversityUNSPECIFIED
NSFIOS-1931114
Subject Keywords:Apical initial, Cell division, Cell size, Ceratopteris richardii, Fern gametophyte, Lateral meristem, Live imaging, Multicellular meristem, Pteris vittata, Seed-free plants
DOI:10.1093/jxb/erab307
Record Number:CaltechAUTHORS:20210621-170112415
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210621-170112415
Official Citation:Xiao Wu, An Yan, Scott A M McAdam, Jo Ann Banks, Shaoling Zhang, Yun Zhou, Timing of meristem initiation and maintenance determines the morphology of fern gametophytes, Journal of Experimental Botany, 2021;, erab307, https://doi.org/10.1093/jxb/erab307
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:109505
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jun 2021 17:32
Last Modified:12 Jul 2021 23:09

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