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A Mutation of lips-6 in C. elegans

Cohen, Sarah M. and Le, Henry H. and Sternberg, Paul W. (2021) A Mutation of lips-6 in C. elegans. microPublication Biology . Art. No. 000419. ISSN 2578-9430. PMCID PMC8271349. doi:10.17912/micropub.biology.000419. https://resolver.caltech.edu/CaltechAUTHORS:20210714-172910613

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Abstract

The efficient CRISPR/Cas9 genome editing toolkit in the nematode Caenorhabditis elegans has made the creation of null mutants easier than ever. The putative lipid hydrolase lips-6 is known to function in the DAF-12/DIN-1 pathway to promote fat mobilization during periods of starvation in adult worms (Tao et al. 2016). Similarly, it was found to be strongly downregulated in response to dauer ascarosides given during the L1 to L2d decision in larval worms (Cohen et al. 2021). Here, we have created a mutant of the putative lipase lips-6 and studied its lifespan as well as its ascaroside profile.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.17912/micropub.biology.000419DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271349PubMed CentralArticle
ORCID:
AuthorORCID
Cohen, Sarah M.0000-0002-5233-2280
Le, Henry H.0000-0003-2942-2357
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:© 2021 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. History: Received June 9, 2021 Revision received July 3, 2021 Accepted July 5, 2021 Published July 9, 2021. We thank Heenam Park and Frank Schroeder for their helpful discussion and comments. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE 1745301 to SMC and Grant No. R24OD023041 to PWS. Author Contributions: Sarah M Cohen: Conceptualization, Investigation, Methodology, Writing - original draft, Visualization. Henry H Le: Methodology, Visualization, Writing - review and editing, Investigation. Paul W Sternberg: Funding acquisition, Project administration, Resources, Supervision, Writing - review and editing. Reviewed By: Jennifer Watts.
Group:WormBase
Funders:
Funding AgencyGrant Number
NSF Graduate Research FellowshipDGE-1745301
NIHR24OD023041
PubMed Central ID:PMC8271349
DOI:10.17912/micropub.biology.000419
Record Number:CaltechAUTHORS:20210714-172910613
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210714-172910613
Official Citation:Cohen, SM; Le, HH; Sternberg, PW (2021). A Mutation of lips-6 in C. elegans. microPublication Biology. https://doi.org/10.17912/micropub.biology.000419
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:109818
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:14 Jul 2021 18:09
Last Modified:14 Jul 2021 18:31

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