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Atp13a5 Marker Reveals Pericytes of The Central Nervous System in Mice

Guo, Xinying and Ge, Tenghuan and Xia, Shangzhou and Wu, Haijian and Colt, Mark and Xie, Xiaochun and Zhang, Bangyan and Zeng, Jianxiong and Chen, Jian-Fu and Zhu, Donghui and Montagne, Axel and Gao, Fan and Zhao, Zhen (2021) Atp13a5 Marker Reveals Pericytes of The Central Nervous System in Mice. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20210720-203604788

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Abstract

Perivascular mural cells including vascular smooth cells (VSMCs) and pericytes are integral components of the vascular system. In the central nervous system (CNS), pericytes are also known as the guardian of the blood-brain barrier, blood-spinal cord barrier and blood-retinal barrier, and play key roles in maintaining cerebrovascular and neuronal functions. However, the functional difference between CNS and peripheral pericytes has not been resolved at the genetic and molecular levels. Hence, the generation of reliable CNS pericyte-specific models and genetic tools remains very challenging. Here, we report a new CNS pericyte marker in mice. This cation-transporting ATPase 13A5 (Atp13a5) marker is highly specific to the pericytes in brain, spinal cord and retina. We generated a transgenic model with a knock-in tdTomato reporter and Cre recombinase. The tdTomato reporter reliably labels the CNS pericytes, but not found in any other CNS cell types including closely related VSMCs, or in peripheral organs. More importantly, Atp13a5 is turned on at embryonic day E15, suggesting brain pericytes are shaped by the developing neural environment. We hope that the new tools will allow us to further explore the heterogeneity of pericytes and achieve a better understanding of CNS pericytes in health and diseases.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2021.07.09.451694DOIDiscussion Paper
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. This version posted July 10, 2021. The work of Z.Z. is supported by the National Institutes of Health (NIH) grant nos. R01AG061288, R01NS110687 and RF1NS122060, and BrightFocus Foundation grant no. A2019218S. Author contributions: XG and ZZ designed and performed experiments and analyzed data and wrote the manuscript. TG, SX, HW, MC, XX, BZ and FG performed experiments and/or analyzed data. JZ, JFC, DZ, AM and FG contributed to writing the manuscript. All authors read and approved the final manuscript. The authors have declared no competing interest.
Funders:
Funding AgencyGrant Number
NIHR01AG061288
NIHR01NS110687
NIHRF1NS122060
BrightFocus FoundationA2019218S
Record Number:CaltechAUTHORS:20210720-203604788
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210720-203604788
Official Citation:Atp13a5 Marker Reveals Pericytes of The Central Nervous System in Mice. Xinying Guo, Tenghuan Ge, Shangzhou Xia, Haijian Wu, Mark Colt, Xiaochun Xie, Bangyan Zhang, Jianxiong Zeng, Jian-Fu Chen, Donghui Zhu, Axel Montagne, Fan Gao, Zhen Zhao. bioRxiv 2021.07.09.451694; doi: https://doi.org/10.1101/2021.07.09.451694
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:109939
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:21 Jul 2021 16:20
Last Modified:21 Jul 2021 16:20

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