A Caltech Library Service

Altered microbial CAZyme families indicated dead biomass decomposition following afforestation

Ren, Chengjie and Zhang, Xinyi and Zhang, Shuohong and Wang, Jieying and Xu, Miaoping and Guo, Yaoxin and Wang, Jun and Han, Xinhui and Zhao, Fazhu and Yang, Gaihe and Doughty, Russell (2021) Altered microbial CAZyme families indicated dead biomass decomposition following afforestation. Soil Biology and Biochemistry, 160 . Art. No. 108362. ISSN 0038-0717. doi:10.1016/j.soilbio.2021.108362.

[img] PDF - Accepted Version
See Usage Policy.

[img] MS Word (Supplementary data) - Supplemental Material
See Usage Policy.


Use this Persistent URL to link to this item:


Afforestation can modify terrestrial carbon (C) pools, some of which are primarily fixed in the plant dead biomass and then incorporated into the microbial dead biomass. Soil microorganisms exert a critical role in C flow and potentially influence C balance through the degradation of plant and microbial dead biomass. Here, we compared sites along a 45-year Robinia pseudoacacia (RP) afforestation chronosequence on the Loess Plateau of China. Subsequently, the trends of microbial carbohydrate-active enzymes (CAZyme) and their responses to the decomposition of dead biomass of different origins were studied using metagenomics. The results show that soil microbial CAZyme families, which degrade the plant- and microbial-derived components, significantly increased after afforestation, with a significant peak at the 20-year site. The dominant bacterial phyla (i.e., Actinobacteria, Proteobacteria, and Acidobacteria) mineralized C sources from plant and microbial biomass components through their corresponding CAZyme families. Moreover, the increased abundance of CAZymes involved in the decomposition of plant-derived components (e.g., cellulose, hemicellulose, and lignin) contributed to the formation of C pools. In the case of microbial-derived components, the abundance of CAZymes encoding the bacterial-derived components (peptidoglycan) was larger than that encoding fungal-derived components (chitin and glucans) and was more associated with microbial metabolic activity (qCO2 and Cmic: Corg ratio), indicating a higher investment of bacterial-derived components for microbial carbon turnover following afforestation. Overall, our study compares plant- and microbial-derived biomass to illustrate the differential contributions of dead biomass to C accumulation and confirms the importance of the bacterial community and derived biomass for C turnover following afforestation.

Item Type:Article
Related URLs:
URLURL TypeDescription
Zhang, Shuohong0000-0002-7482-8148
Doughty, Russell0000-0001-5191-2155
Additional Information:© 2021 Elsevier Ltd. Received 21 February 2021, Revised 15 July 2021, Accepted 16 July 2021, Available online 27 July 2021. This work were financially supported by the National Natural Science Foundation of China (No. 41907031), Shaanxi Innovation Capability Support Program (2019PT-13), the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2020JQ-272; 2019JM-211), the 2021 first funds for central government to guide local science and technology development in Qinghai Province (2021ZY002), the Young Talents Support Program of the Science and Technology Association of Shaanxi Provincial Universities (No. 20200701), the Chinese Academy of Sciences “Light of West China” Program for Introduced Talent in the West (No. 31570440) and the China Postdoctoral Science Foundation (No. 2019M650276). The authors are also grateful to anonymous reviewers whose comments and suggestions helped us to enhance the quality of this paper. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The authors declare no competing financial interest.
Funding AgencyGrant Number
National Natural Science Foundation of China41907031
Shaanxi Innovation Capability Support Program2019PT-13
Natural Science Basic Research Plan in Shaanxi Province of China2020JQ-272
Natural Science Basic Research Plan in Shaanxi Province of China2019JM-211
Qinghai Province2021ZY002
Shaanxi Provincial Universities20200701
Chinese Academy of Sciences31570440
China Postdoctoral Science Foundation2019M650276
Subject Keywords:Metagenomics; Microbial CAZyme families; Plant-derived components; Microbial-derived components; Microbial metabolic activity; Afforestation
Record Number:CaltechAUTHORS:20210820-002434428
Persistent URL:
Official Citation:Chengjie Ren, Xinyi Zhang, Shuohong Zhang, Jieying Wang, Miaoping Xu, Yaoxin Guo, Jun Wang, Xinhui Han, Fazhu Zhao, Gaihe Yang, Russell Doughty, Altered microbial CAZyme families indicated dead biomass decomposition following afforestation, Soil Biology and Biochemistry, Volume 160, 2021, 108362, ISSN 0038-0717,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:110319
Deposited By: Tony Diaz
Deposited On:20 Aug 2021 16:52
Last Modified:20 Aug 2021 16:52

Repository Staff Only: item control page