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Protein profiling in the habenula after chronic (–)-menthol exposure in mice

Mulcahy, Matthew J. and Huard, Stephanie M. and Paulo, Joao A. and Wang, Jonathan H. and McKinney, Sheri and Marks, Michael J. and Henderson, Brandon J. and Lester, Henry A. (2021) Protein profiling in the habenula after chronic (–)-menthol exposure in mice. Journal of Neurochemistry, 158 (6). pp. 1345-1358. ISSN 0022-3042. doi:10.1111/jnc.15495.

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The identification of proteins that are altered following nicotine/tobacco exposure can facilitate and positively impact the investigation of related diseases. In this report, we investigated the effects of chronic (–)-menthol exposure in 14 murine brain regions for changes in total β2 subunit protein levels and changes in epibatidine binding levels using immunoblotting and radioligand binding assays. We identified the habenula as a region of interest due to the region's marked decreases in β2 subunit and nAChR levels in response to chronic (–)-menthol alone. Thus, we further examined the habenula, a brain region associated with both the reward and withdrawal components of addiction, for additional protein level alterations using mass spectrometry. A total of 552 proteins with altered levels were identified after chronic (–)-menthol exposure. Enriched in the proteins with altered levels after (–)-menthol exposure were proteins associated with signaling, immune systems, RNA regulation, and protein transport. The continuation and expansion of the brain region-specific protein profiling in response to (–)-menthol will provide a better understanding of how this common flavorant in tobacco and e-liquid products may affect addiction and general health.

Item Type:Article
Related URLs:
URLURL TypeDescription
Mulcahy, Matthew J.0000-0001-5588-8762
Huard, Stephanie M.0000-0002-2333-1852
Paulo, Joao A.0000-0002-4291-413X
Henderson, Brandon J.0000-0003-0381-028X
Lester, Henry A.0000-0002-5470-5255
Additional Information:© 2021 International Society for Neurochemistry. Issue Online: 22 September 2021; Version of Record online: 02 September 2021; Accepted manuscript online: 18 August 2021; Manuscript accepted: 11 August 2021; Manuscript revised: 10 July 2021; Manuscript received: 02 March 2021. Research Funding: National Institutes of Health. Grant Numbers: DA036061, DA040047, DK098285.
Funding AgencyGrant Number
Subject Keywords:addiction; immunoblotting; mass spectrometry; menthol; nicotine; radioligand binding
Issue or Number:6
Record Number:CaltechAUTHORS:20210820-224447031
Persistent URL:
Official Citation:Mulcahy, M. J., Huard, S. M., Paulo, J. A., Wang, J. H., McKinney, S., Marks, M. J., Henderson, B. J., & Lester, H. A. (2021). Protein profiling in the habenula after chronic (–)-menthol exposure in mice. Journal of Neurochemistry, 158, 1345– 1358.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:110334
Deposited By: Tony Diaz
Deposited On:21 Aug 2021 17:47
Last Modified:05 Oct 2021 23:32

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