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A novel widespread bacterial structure related to the flagellar type III secretion system

Kaplan, Mohammed and Oikonomou, Catherine M. and Wood, Cecily R. and Chreifi, Georges and Ghosal, Debnath and Dobro, Megan J. and Yao, Qing and McDowall, Alasdair and Briegel, Ariane and Beeby, Morgan and Chang, Yi-Wei and Shaffer, Carrie L. and Jensen, Grant J. (2021) A novel widespread bacterial structure related to the flagellar type III secretion system. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20210907-221825591

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Abstract

The flagellar type III secretion system (fT3SS) is a suite of membrane-embedded and cytoplasmic proteins responsible for building the bacterial flagellar motility machinery. Homologous proteins form the injectisome machinery bacteria use to deliver effector proteins into eukaryotic cells, and other family members have recently been reported to be involved in the formation of membrane nanotubes. Here we describe a novel, ubiquitous and evolutionarily widespread hat-shaped structure embedded in the inner membrane of bacteria, of yet-unidentified function, that is related to the fT3SS, adding to the already rich repertoire of this family of nanomachines.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2021.09.03.458937DOIDiscussion Paper
ORCID:
AuthorORCID
Kaplan, Mohammed0000-0002-0759-0459
Oikonomou, Catherine M.0000-0003-2312-4746
Chreifi, Georges0000-0003-4194-1694
Ghosal, Debnath0000-0002-2227-0330
Dobro, Megan J.0000-0002-6464-3932
Yao, Qing0000-0003-3575-9909
Briegel, Ariane0000-0003-3733-3725
Beeby, Morgan0000-0001-6413-9835
Chang, Yi-Wei0000-0003-2391-473X
Jensen, Grant J.0000-0003-1556-4864
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This version posted September 4, 2021. This project was funded by the NIH (grant R01 AI127401 to G.J.J., and P20 GM130456 and P30 GM110787 to C.L.S.) and a Baxter postdoctoral fellowship from Caltech to M.K. Cryo-ET work was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at the California Institute of Technology. We are grateful to Prof. Marc Erhardt (Humboldt-Universitat zu Berlin) for critically reading an initial version of this work. We thank Prof. Elitza I. Tocheva for collecting the A. tumefaciens data, Dr. Jian Shi for collecting the H. neptunium data, and Prof. Martin Pilhofer for collecting the P. luteoviolacea data.
Funders:
Funding AgencyGrant Number
NIHR01 AI127401
NIHP20 GM130456
NIHP30 GM110787
Baxter FoundationUNSPECIFIED
DOI:10.1101/2021.09.03.458937
Record Number:CaltechAUTHORS:20210907-221825591
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210907-221825591
Official Citation:A novel widespread bacterial structure related to the flagellar type III secretion system. Mohammed Kaplan, Catherine M Oikonomou, Cecily R. Wood, Georges Chreifi, Debnath Ghosal, Megan J Dobro, Qing Yao, Alasdair McDowall, Ariane Briegel, Morgan Beeby, Yi-Wei Chang, Carrie L. Shaffer, Grant J. Jensen. bioRxiv 2021.09.03.458937; doi: https://doi.org/10.1101/2021.09.03.458937
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:110754
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:08 Sep 2021 15:41
Last Modified:16 Nov 2021 19:42

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