A Caltech Library Service

Entry to and exit from diapause arrest in Caenorhabditis elegans are both regulated by a steroid hormone pathway

Zhang, Mark G. and Sternberg, Paul W. (2021) Entry to and exit from diapause arrest in Caenorhabditis elegans are both regulated by a steroid hormone pathway. . (Unpublished)

[img] PDF - Submitted Version
Creative Commons Attribution.


Use this Persistent URL to link to this item:


Diapause arrest in animals such as Caenorhabditis elegans is tightly regulated so that animals make appropriate developmental decisions amidst environmental challenges. Fully understanding diapause requires mechanistic insight of both entry and exit from the arrested state. While a steroid hormone pathway regulates the entry decision into Caenorhabditis elegans dauer diapause, its role in the exit decision is less clear. A complication to understanding steroid hormonal regulation of dauer has been the peculiar fact that steroid hormone mutants such as daf-9 form partial dauers under normal growth conditions. Here, we corroborate previous findings that daf-9 mutants remain capable of forming full dauers under unfavorable growth conditions, and we establish that the daf-9 partial dauer state is likely a partially exited dauer that has initiated but cannot complete the dauer exit decision. We show that the steroid hormone pathway is both necessary for and promotes complete dauer exit, and that the spatiotemporal dynamics of steroid hormone regulation during dauer exit resembles that of dauer entry. Overall, dauer entry and dauer exit are distinct developmental decisions that are both controlled by steroid hormone signaling.

Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription Paper
Zhang, Mark G.0000-0002-0802-300X
Sternberg, Paul W.0000-0002-7699-0173
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license. This version posted September 6, 2021. Δ7-DA was a gift from the lab of Frank Schroeder (Cornell University). We thank Mengyi Cao and Stephanie Nava for the XXX cGAL driver. Some strains were provided by the CGC, which is funded by NIH Office of Research Infrastructure Programs (P40 OD010440). We are grateful to members of the Sternberg lab for their feedback on the manuscript, particularly Hillel Schwartz. M.G.Z. was funded by National Institutes of Health Grant F31 NS120501-01. A National Institutes of Health Grant UF1-NS111697 (P.W.S) supported the research material and research assistants. Author Contributions: M.G.Z. and P.W.S. conceived of the study. M.G.Z. performed the experiments and analyzed the data. M.G.Z. wrote the manuscript with editorial assistance from P.W.S. No competing interests declared.
Funding AgencyGrant Number
NIH Postdoctoral FellowshipF31 NS120501-01
NIHP40 OD010440
Subject Keywords:dauer, diapause, hormone, decision, daf-9, daf-12
Record Number:CaltechAUTHORS:20210910-174112976
Persistent URL:
Official Citation:Entry to and exit from diapause arrest in Caenorhabditis elegans are both regulated by a steroid hormone pathway. Mark G Zhang, Paul W Sternberg. bioRxiv 2021.09.04.458989; doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:110804
Deposited By: Tony Diaz
Deposited On:10 Sep 2021 20:16
Last Modified:16 Nov 2021 19:42

Repository Staff Only: item control page