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Chronic exposure to odors at naturally occurring concentrations triggers limited plasticity in early stages of Drosophila olfactory processing

Gugel, Zhannetta V. and Maurais, Elizabeth and Hong, Elizabeth J. (2021) Chronic exposure to odors at naturally occurring concentrations triggers limited plasticity in early stages of Drosophila olfactory processing. . (Unpublished) https://resolver.caltech.edu/CaltechAUTHORS:20210910-223730081

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Abstract

In insects and mammals, chronic exposure to odors at high concentrations in early life alters olfactory function, but the role of odor experience-dependent plasticity in more naturalistic contexts is less clear. We investigated olfactory plasticity in the Drosophila antennal lobe by exposing flies to odors at concentrations that are typically encountered in natural odor sources. These stimuli also strongly and selectively activated only a single class of olfactory receptor neuron (ORN) input, facilitating the investigation of input-specific plasticity. Overall, chronic exposure to three such odors elicited limited plasticity in the odor responses of second-order projection neurons (PNs). Exposure to some odors elicited mild increases in PN responses to weak stimuli, extending the lower bound of the dynamic range of PN signaling. When present, plasticity was observed broadly in multiple PN types and thus was not selective for PNs receiving direct input from the chronically active ORNs. Chronic E2-hexenal exposure did not affect PN intrinsic properties, local inhibitory innervation, ORN responses, or ORN-PN synaptic strength, but modestly increased broad lateral excitation evoked by some odors. These results show that PN odor coding is only mildly affected by strong persistent activation of a single olfactory input and highlight the stability of early stages of insect olfactory processing to significant perturbations in the sensory environment.


Item Type:Report or Paper (Discussion Paper)
Related URLs:
URLURL TypeDescription
https://doi.org/10.1101/2021.09.03.458834DOIDiscussion Paper
ORCID:
AuthorORCID
Hong, Elizabeth J.0000-0003-3866-418X
Additional Information:The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This version posted September 3, 2021. We thank Chris Potter for the gift of Or7a-KI flies. We thank Meike Lobb-Rabe for generating the Or7a-lexA flies. We thank Daniel Wagenaar and the Caltech Neurotechnology Center for technical support in constructing fly holders and custom odor delivery devices. We thank members of the Hong laboratory for their critical reading and feedback on this manuscript. This work was supported by grants from the National Science Foundation (Award #1556230), the Shurl and Kay Curci Foundation, and the National Institutes of Health (1RF1MH117825). E.J.H. was supported by a Clare Boothe Luce professorship. The authors declare no competing interests.
Group:Tianqiao and Chrissy Chen Institute for Neuroscience
Funders:
Funding AgencyGrant Number
NSFIOS-1556230
Shurl and Kay Curci FoundationUNSPECIFIED
NIH1RF1MH117825
Clare Boothe Luce ProfessorshipUNSPECIFIED
DOI:10.1101/2021.09.03.458834
Record Number:CaltechAUTHORS:20210910-223730081
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20210910-223730081
Official Citation:Chronic exposure to odors at naturally occurring concentrations triggers limited plasticity in early stages of Drosophila olfactory processing. Zhannetta V. Gugel, Elizabeth Maurais, Elizabeth J. Hong. bioRxiv 2021.09.03.458834; doi: https://doi.org/10.1101/2021.09.03.458834
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:110816
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:13 Sep 2021 18:54
Last Modified:16 Nov 2021 19:42

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