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Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate

Snopek, Thomas J. and Wood, William B. and Conley, M. Patricia and Chen, Peter and Cozzarelli, Nicholas R. (1977) Bacteriophage T4 RNA ligase is gene 63 product, the protein that promotes tail fiber attachment to the baseplate. Proceedings of the National Academy of Sciences of the United States of America, 74 (8). pp. 3355-3359. ISSN 0027-8424.

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RNA ligase and tail fiber attachment activities, normally induced following bacteriophage T4 infection of Escherichia coli, are not induced when gene 63 amber mutants of T4 infect nonpermissive host cells. Both activities are induced when these mutants infect permissive hosts, or when revertants of these mutants infect nonpermissive hosts. When one of these mutants infects a host that carries supF, both activities are more than normally heat labile. RNA ligase, purified to homogeneity, promotes the tail fiber attachment reaction in vitro with a specific activity similar to that of the most highly purified preparations of gene 63 product isolated on the basis of tail fiber attachment activity. We conclude that T4 RNA ligase is gene 63 product. The RNA ligase and tail fiber attachment reactions differ in requirements and in response to some inhibitors, suggesting that the two activities of the gene 63 product may be mechanistically unrelated.

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Additional Information:© 1977 by the National Academy of Sciences. Contributed by William B. Wood, June 10, 1977. We thank S.A. Rashbaum for expert technical assistance. This research was supported by grants from the U.S. Public Health Service to N.R.C. (GM-22729) and W.B.W. (AI-09238). The costs of publication of this article were defrayed in part by the payment of page charges from funds made available to support the research which is the subject of the article. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Funding AgencyGrant Number
Public Health ServiceGM-22729
Public Health ServiceAI-09238
Issue or Number:8
Record Number:CaltechAUTHORS:SNOpnas77
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11090
Deposited By: Tony Diaz
Deposited On:16 Jul 2008 19:38
Last Modified:03 Oct 2019 00:15

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