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Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis

Kaplan, Mohammed and Tocheva, Elitza I. and Briegel, Ariane and Dobro, Megan J. and Chang, Yi-Wei and Subramanian, Poorna and McDowall, Alasdair and Beeby, Morgan and Jensen, Grant J. (2021) Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis. mBio, 12 (3). Art. No. mBio.00298-21. ISSN 2150-7511. PMCID PMC8263016. doi:10.1128/mBio.00298-21. https://resolver.caltech.edu/CaltechAUTHORS:20211006-155256213

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Abstract

The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other components of the motor. This loss may be prevented by the formation of a cytoplasmic vesicle around the complex. These observations suggest a relatively loose association of the switch complex with the rest of the flagellar machinery.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1128/mBio.00298-21DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc8263016/PubMed CentralArticle
ORCID:
AuthorORCID
Kaplan, Mohammed0000-0002-0759-0459
Tocheva, Elitza I.0000-0002-4869-8319
Briegel, Ariane0000-0003-3733-3725
Dobro, Megan J.0000-0002-6464-3932
Chang, Yi-Wei0000-0003-2391-473X
Beeby, Morgan0000-0001-6413-9835
Jensen, Grant J.0000-0003-1556-4864
Additional Information:© 2021 Kaplan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received 5 February 2021; Accepted 26 April 2021; Published 8 June 2021. This project was funded by the NIH (grant RO1 AI127401 to G.J.J.) and a Baxter postdoctoral fellowship from Caltech to M.K. Cryo-ET work was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at the California Institute of Technology. We are grateful to Catherine Oikonomou for critically reading the manuscript. We thank Cristina Iancu for collecting H. crunogenus data.
Funders:
Funding AgencyGrant Number
NIHRO1 AI127401
Baxter FoundationUNSPECIFIED
Issue or Number:3
PubMed Central ID:PMC8263016
DOI:10.1128/mBio.00298-21
Record Number:CaltechAUTHORS:20211006-155256213
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20211006-155256213
Official Citation:Kaplan M, Tocheva EI, Briegel A, Dobro MJ, Chang Y-W, Subramanian P, McDowall AW, Beeby M, Jensen GJ. 2021. Loss of the bacterial flagellar motor switch complex upon cell lysis. mBio 12:e00298-21. https://doi.org/10.1128/mBio.00298-21
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:111237
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Oct 2021 16:33
Last Modified:06 Oct 2021 16:33

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