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Mutations affecting sexual development in Phycomyces blakesleeanus

Sutter, Richard P. (1975) Mutations affecting sexual development in Phycomyces blakesleeanus. Proceedings of the National Academy of Sciences of the United States of America, 72 (1). pp. 127-130. ISSN 0027-8424.

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Although zygospore (mature zygote) formation in P. blakeslleeanus occurs in liquid glucoseglutamate medium, morphological observations are made more easily when cultures are grown on 1-mm-thick agar medium. Zygophores (sexually differentiated hyphae) develop prior to physical contact in crosses of (plus) and (minus) wild types. Zygophores interlock upon contact and then undergo six successive morphological changes to become a zygospore. Mutants with abnormal carotene synthesis exhibit aberrant sexual behavior. Some zygospores do form in crosses of carA mutants and wild types. Only paired zygophores form in crosses of wild type(plus) with car-42(minus), a beta-carotene-accumulating mutant. Zygophores form only on (minus) in crosses of wild type(plus) with carB(minus), carR(minus), carAcarR(minus), and carBcarR(minus) mutants, and only on (plus) in crosses of car-43(plus) with wild type(minus), car-42(minus), and carA(minus) mutants. Zygophores do not form in crosses of car-43(plus) with carB(minus), carR(minus), carAcarR(minus), and carBcarR(minus) mutants. These observations demonstrate that each mating type makes a chemical messenger that stimulates zygophore development in the opposite mating type.

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Additional Information:© 1975 by the National Academy of Sciences. Communicated by Max Delbrück, October 30, 1974. I thank M. Delbrück, A. Eslava, and H. K. Mitchell for many helpful discussions, M.D. and H.K.M. for providing laboratory space, S. Benzer for use of a photomicroscope, D.F. Ready and P.F. Koen for lessons in photography, and J. Navest for providing technical assistance. This work was done in part during sabbatical leave at the California Institute of Technology and was supported by NSF Grants GB-26636 to M.D. and GB-25033.
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National Science FoundationGB-26636
National Science FoundationGB-25033
Subject Keywords:car mutants; defined medium
Record Number:CaltechAUTHORS:SUTpnas75
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:11146
Deposited By: Tony Diaz
Deposited On:19 Jul 2008 06:22
Last Modified:14 Nov 2014 19:20

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