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Localization of actin in Dictyostelium amebas by immunofluorescence

Eckert, Barry S. and Lazarides, Elias (1978) Localization of actin in Dictyostelium amebas by immunofluorescence. Journal of Cell Biology, 77 (3). pp. 714-721. ISSN 0021-9525. PMCID PMC2110147. doi:10.1083/jcb.77.3.714. https://resolver.caltech.edu/CaltechAUTHORS:ECKjcb78

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Abstract

Antibody prepared against avian smooth muscle actin has been used to localize actin in the slime mold, Dictyostelium discoideum. The distribution of actin in migrating cells is different from that in feeding cells. Migrating amebas display fluorescence primarily in advancing regions whereas feeding amebas show uniform fluorescence throughout. The reaction is specific for actin since the fluorescence observed is blocked when the antibody is absorbed by actin purified from avian skeletal muscle, human platelets, and Dictyostelium. These results, in addition to describing the distribution of actin in D. discoideum, demonstrate that actins from these diverse sources share at least one common antigenic determinant.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1083/jcb.77.3.714DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/pmc2110147/PubMed CentralArticle
Additional Information:© 1978 by The Rockefeller University Press. RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode. Received for publication 9 December 1976, and in revised form 31 October 1977. The authors wish to express their gratitude to Dr. Keith R. Porter, in whose laboratory this work was done. Dr. Eckert was supported by grant RR 00592 from the National Institutes of Health to Dr. Porter. Dr. Lazarides was supported by a post-doctoral fellowship from the Muscular Dystrophy Association and by grant GM 06965 from the National Institutes of Health.
Funders:
Funding AgencyGrant Number
NIHRR00592
Muscular Dystrophy AssociationUNSPECIFIED
NIHGM 06965
Subject Keywords:cellular slime mold; cell motility; antibody against actin; microfilaments; cell morphology
Issue or Number:3
PubMed Central ID:PMC2110147
DOI:10.1083/jcb.77.3.714
Record Number:CaltechAUTHORS:ECKjcb78
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:ECKjcb78
Usage Policy:RUP grants the public the non-exclusive right to copy, distribute, or display the Work under a Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/ and http://creativecommons.org/licenses/by-nc-sa/3.0/legalcode.
ID Code:11168
Collection:CaltechAUTHORS
Deposited By: Archive Administrator
Deposited On:21 Jul 2008 22:17
Last Modified:08 Nov 2021 21:33

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